Isoetes L. is a genus of lycophytes widely distributed around the world that has a large number of endemic species. Here we document the first successful large scale ex-situ cultivation of the new endemic species from Brazilian Amazon quillworts Isoetes cangae and Isoetes serracarajensis.These isoetids are endemic of an iron mining site and grow on a superficial broad applicability, assisting other low-cost cultivation studies, which are very important in ecosystem recovery of mining areas and conservation strategies.