Objective:: Euphorbia is a plant from the Euphorbiaceae family and it has traditionally been used to treat gonorrheal, respiratory tract infection, skin infections and malaria. The aim of present study was to evaluate of antibacterial, antifungal and antioxidant activities of latex and extracts of Euphorbia monostyla. Materials and Methods:: antimicrobial activity was studied by Disc-well diffusion and MIC-MBC methods, also its antioxidant activity was evaluated by DPPH and FRAP methods. Total phenolic content was evaluated by Folin-Ciocalteu reagent. Results:: It is displayed as a result of work that solvent polarity will play a key role to increase phenolic solubility. Methanol extract gave the highest percentage yield and ethyl acetate resulted to the lowest percentage yield. The antimicrobial test showed that whole extracts had no beneficial uses on microorganisms in compare of antibiotics types. Analysis of antioxidant assays revealed that aqueous and methanol extracts had highest antioxidant properties. Conclusion:: whole extracts had any beneficial uses on microorganisms in compare of antibiotics types but aqueous extract of E. monostyla had moderate antioxidant activity and may contain valuable active compounds. This work is the first study about E. monostyla and further study is suggested to isolate its active compounds.