Flavonoids belong to the largest group of a phenolic compound found in plants. The presence of these flavonoids is significant for the plants themselves such as protecting plants from UV-B, insect pests, biotic, and abiotic stresses. More than 10,000 varieties of flavonoids have been already identified. Several plants can synthesize flavonoids, one of which is ferns. Cosmopolitan ferns are found in tropical and subtropical regions like Baluran National Park. The ecosystem in Baluran National Park has components that can show different sensitivity level to the factors influencing the environment, either in terms of biotic and abiotic factors. Same species of plants in different environments can be different significantly in their secondary metabolite content. The primary purpose of this research was to determine the types of flavonoids ferns in Baluran National Park. This study was conducted in April-June 2018. The extraction of samples was conducted using 96% methanol solvent and the analysis using the thin layer chromatography. The results showed the fourth type of flavonoid rutin, hyperoside, quercitrin, and quercertin contained in Pseudocyclosorrus ochthodes (Kunze) Holttum, Phymatodes scolopendria (Burm.) Ching. Stenochlaena palustris (Burm.), except routine not found in Dryopteris hirtipes (Bl.) Kuntze Beddome. Pteris vittata L. does not contain all four types. Types of flavonoids from ferns in Baluran National Park are routine, hyperoxide, quercitrin and quercetin.

Secondary metabolites of fern are increasingly recognized as medicine ingredient. The total of secondary metabolites in plants may form maximally related to the altitude, environment, nutrient, biotic and abiotic stresses. Dryopteris hirtipes (Bl.) Kuntze is easily found in Baluran National Park and Raden Soerjo Grand Forest Park. This study aimed to investigate the differences between secondary metabolites of Dryopteris hirtipes (Bl.) Kuntze from Baluran National Park and Raden Soerjo Grand Forest Park. The samples were taken from Baluran National Park which has a height of 0-1000 masl, with a rainfall intensity about 2000-4000 mm per year, and Raden Soerjo Forest Park which has an altitude of 1000-3000 masl, with rainfall intensity 1488 mm per year. The research was conducted by Thin Layer Chromatography (TLC) method. Samples of Dryopteris hirtipes’ rhizome and fronds were made as powder then extracted using methanol (1:10). Methanol leaf extract subsequently tested by TLC test. Both of Dryopteris hirtipes (Bl.) Kuntze from Baluran National Park and Raden Soerjo Grand Forest Park have similarity chemical content of secondary metabolites such as alkaloid, terpenoid-saponin, tannin, but polyphenols only found from Baluran National Park, and flavonoids only found from Raden Soerjo Grand Forest Park.