To solve the cooperation-conflict negotiation problems among the members of High-tech Virtual Enterprise (HTVE), High-tech Virtual Enterprise Negotiation Support System (HTVE-NSS) based on RIA architecture is put forward. According to the operation characteristics of High-tech Virtual Enterprise, in this system, the structure of High-tech Virtual Enterprise Negotiation Support System is designed, which consists of the problem processing system, the model base system and remote conference system. And HTVE-NSS adopts artificial intelligence technology to design problem processing system (PPS), and uses UML-Petri net to describe the process of model. The architecture of Negotiation Conference System based on Flex and RIA is designed and Red5 Streaming media server is introduced to support conference interaction, which effectively supports the way to solve the conflict between High-Tech Virtual Enterprises by negotiating.