In this study, we consider a boundary value problem generated by the Sturm-Liouville equation with a frozen argument and with non-separated boundary conditions on a time scale. Firstly, we present some solutions and the characteristic function of the problem on an arbitrary bounded time scale. Secondly, we prove some properties of eigenvalues and obtain a formulation for the eigenvalues-number on a finite time scale. Finally, we give an asymptotic formula for eigenvalues of the problem on another special time scale: $\mathbb{T}=[\alpha,\delta_{1}]\bigcup[\delta_{2},\beta].$