Today, almost 2 million new leishmaniasis cases are noted annually; 1.5 million of these are cutaneous (CL), and others are visceral leishmaniasis (VL). In Sanliurfa, CL cases caused by Leishmania tropica but not by other agents such as L. infantum and L. major. L. tropica is a unique parasite species in Sanliurfa and is the causative agent of anthroponotic CL (transmitted from human to vector to human). Our aim was to report 3 new CL cases due to L. major ( 2 autochthonous and 1 imported) identified in Sanliurfa. Lesion aspiration samples taken from patients were inoculated into NNN culture. Following successful isolation in NNN, promastigotes were obtained by mass culture using RPMI + 20% FCS medium. Parasites species were identified as L. major using ITS-1 PCR-RFLP analysis. This is the first report of autochthonous CL cases caused by L. major in Sanliurfa, and it is estimated that the number of such cases will increase in this region. Public health measures should be taken for L. major infections, while researchers should plan field studies to identify the vectors and reservoirs of L. major.