ФГБУ «Научно-исследовательский институт гриппа» Минздрава России, 197376, г. Санкт-Петербург В работе изучена этиологическая структура гриппа и других ОРВи в Санкт-Петербурге и Ленинградской области, установлена их доля в течение 4 эпидемических сезонов. Показана сезонность некоторых респираторных вирусов и обозначены пики циркуляции респираторно-синцитиального (РС) вируса, аденовируса, вирусов парагриппа, риновируса, бокавируса, метапневмовируса и коронавируса. Прослежено взаимовлияние вирусов гриппа А и РС-вирусов, РС-вирусов и риновирусов. Выявлен высокий уровень заболеваемости аденовирусной инфекцией в организованных коллективах и РС-вирусной инфекцией у детей. К л ю ч е в ы е с л о в а: вирусы гриппа; острые респираторные вирусные инфекции; ПЦР-диагностика; эпидемический сезон. The etiological structure of influenza and other acute respiratory viral infections including their rate of incidence in St. Petersburg and Leningrad region during 4 epidemic seasons has been studied. Seasonality of some respiratory viruses was shown and peaks of circulation of RSV, adenovirus, parainfluenza viruses, rhinovirus, bocavirus, metapneumovirus and coronavirus were marked. The interference of influenza A viruses and RSV, RSV and rhinoviruses was highlighted. A high incidence of adenovirus infection in organized communities and RSV infection in children was revealed. K e y w o r d s: Influenza viruses; acute respiratory viral infections (ARVI); PCR diagnostics; epidemic season. For citation: Pisareva M.M., Eder V.A., Buzitskaya Zh.V., Musaeva T.D., Afanaseva V.S., Go A.A., Obraztsova E.A., Sukhovetskaya V.F., Komissarov A.B. Etiological structure of influenza and other ARVI in St. Petersburg during epidemic seasons Information about authors: Pisareva M.M., http://orcid.org/0000-0002-1499-9957Eder V.A., http://orcid.org/0000-0002-9970-3325 Buzitskaya Zh.V., http://orcid.org/0000-0002-8394-102X Musaeva T.D., https://orcid.org/0000-0002-3050-1936 Afanasieva V.S., http://orcid.org/0000-0002-2887-0126 Sukhovetskaya V.F., http://orcid.org/0000-0002-1566-7137 Komissarov A.B., http://orcid.org/Для корреспонденции: Писарева Мария Михайловна, канд. биол. наук, ведущий научный сотрудник лаборатории молекулярной вирусологии ФГБУ «НИИ гриппа» Минздрава России, 197376, г. Санкт-Петербург.

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Pseudomonads' IncP-7 plasmids make significant contribution to the environmental biodegradative potential and sometimes harbour antibiotic resistance genes. More than 30 years plasmid Rms148 is used as archetypal P-7 plasmid in microbiological incompatibility tests. However, the structure of its basic replicon was not described up to now, as well as phylogenetic relationships between all known plasmids within the IncP-7 group were not studied. In the frames of this work we have constructed two primer pairs to amplify main components of P-7 replication initiation region, and subsequent screening of repA intragenic polymorphism was made using laboratory collection of IncP-7 plasmids. Minimal replicon of Rms148 was constructed and its nucleotide sequence was determined to be identical to repA-oriVof known P-7 plasmids on 81-83% and forming separate branch on appropriate phylogenetic tree. Additionally, repA seems to be more conservative between group members compared with putative oriV region. Deduced amino acid sequence and predicted secondary and tertiary structures of Rms148 RepA protein allow us to make assumption about similar to unclassified cryptic plasmid pPS10 model of replication initiation for IncP-7 group members.