“Huangjinya” is a light-sensitive albino variety and is widely cultivated in China. It has been proved that red light could promote the vegetable growth of plants. However, the mechanism of “Huangjinya” in response to a red light is unclear. This study used high-throughput sequencing technology to analyze the transcriptome of tender shoots of “Huangjinya” under the white and red light supplement conditions. At the same time, liquid chromatography tandem mass spectrometry (LC-MS) was used to analyze metabolite changes under different light conditions. Transcriptome analysis revealed that a total of 174 differentially expressed genes (DEGs) were identified after the red light supplement. Kyoto encyclopedia of genes and genomes (KEGG) classification indicated that amino acid metabolism enriched the most DEGs. In addition, two phenylpropanoid metabolism-related genes and five glutathione S-transferase genes (CsGSTs) were found to be expressed differently. Metabolome analysis revealed that 193 differential metabolites were obtained. Being the same as transcriptome analysis, most differential metabolites were enriched in amino acids, sweet and umami tasting amino acids were increased, and bitter-tasting amino acids were decreased after the red light supplement. In summary, red light supplementary treatment may be propitious to the quality of “Huangjinya” due to its regulatory effect on amino acid metabolism. Also, CsGSTs involved phenylpropanoid metabolism contributed to tea quality changes in “Huangjinya.”

Pea-tea intercropping is an excellent cultivation method that can improve tea quality. However, the underlying mechanism is still unclear. The present study was aimed at elucidating the mechanism of the effect of pea-tea intercropping on tea quality through a high-throughput method. Transcriptome and metabolome analyses were conducted to identify the changes in gene expression and metabolites changes intercropping, respectively. In addition, the amino acids and catechins were detected using the LC-MS method and quantified absolutely. The results showed that total polyphenols and catechins decreased but amino acids increased in pea intercropped tea shoots. Correspondingly, genes related to amino acid metabolism and flavonoid biosynthesis were differentially expressed. For amino acid metabolism, 11 differentially expressed genes were identified, including 5 upregulated and 6 downregulated genes. Meanwhile, three genes involved in carbohydrate transport and metabolism were upregulated in pea intercropped tea plants. These genes were also involved in amino acid metabolism. For flavonoid biosynthesis, two downregulated genes were identified, which were the flavonol synthase and anthocyanidin synthase genes and followed a similar pattern to changes in catechins and polyphenols. These advances have opened new horizons for understanding the biochemical mechanisms of amino acids and flavonoids in improving tea quality in the pea-tea intercropping cultivation model.

Background and Aim Pea-tea intercropping is known to improve tea quality but its potential mechanism remains unclear. This study aimed to elucidate the effects of pea-tea intercropping on rhizosphere soil microbial communities and interpret its potential role in the improvement of tea quality. Methods A high-throughput sequencing method was used to detect the microbial communities in the rhizosphere of pea-intercropped tea plants. KEGG and eggNOG databases were used for the functional annotation of microbial DNA sequences. Carbohydrate-active enzymes were used to evaluate soil quality. Results Some microbes in the rhizosphere of pea-intercropped tea plants showed large variations, particularly phyla Acidobacteria and Proteobacteria. The abundance of Bradyrhizobiaceae of Proteobacteria, which help in nitrogen fixation, increased significantly. The annotation of carbohydrate-active enzymes revealed that the relative content of glycoside hydrolases (GHs) increased significantly in the soil microbes of pea-intercropped tea plants. The KEGG analysis showed that more amino acid- and carbohydrate metabolism-related genes were enriched in the soil microbes of pea-intercropped tea plants. To sum up, pea-tea intercropping could improve tea quality by regulating soil microbes in terms of carbon- and nitrogen-fixing capacities.

This paper presents a robot autonomous calibration approach using a probe. The robot grips a simple probe automatically to touch constraint planes in a workspace (the locations of the constraint planes are not necessarily known exactly). The robot internal sensor measurements are recorded for kinematics calibration while the tip-point of the probe is in contact with the constraint plane. The kinematic constraint conditions are obtained from the known shape of the constraint surface, rather than from the measured reference locations in a workspace. This method eliminates any use of external measuring devices, and is suitable for a periodic robot re-calibration. Simulation results on a polishing robot show that this method can achieve the same calibration accuracy as that of the other traditional calibration approach such as geometrical parameter calibration.