Bioadhesives applied on human organs are promising soft implants for interventional diagnostics and therapeutics. However, the conventional bioadhesive interfaces on organs typically lack apical−basal polarity to resemble the surface functions of original organ epithelia. To overcome the bioadhesive-induced dysfunction on treated organs, we have developed an innovative strategy via engineering asymmetric surface functionalities on a tough yet biodegradable polysaccharide-peptide-derived hydrogel platform, mimicking the functions of a ciliated columnar epithelium enabled by its adhesive basal surface and defensive apical ciliated surface. The resulting hydrogel bioadhesive serves as a "stamp" with a polyacrylic acid-functionalized adhesive side, facilitating instant and robust adhesion on wet tissues within 1 min via body liquid-removing mechanisms and Ca 2+ -assisted complexation. The back side is functionalized with hyaluronic acid, demonstrating an outstanding biolubrication performance (coefficient of friction of ∼0.038 in the synovial fluid). The hydrogel stamp can also be integrated with biosensing and drug encapsulation/release functions for diagnostics and therapeutics. Our strategy devises a new path to simultaneously enable reliable wet tissue adhesion and reproduce the characteristics of original tissues, with useful insights into designing bioactive interfaces for broad biomedical applications.