With the proposed "the Belt and Road" strategy in China, the railway logistics specialty becomes one of the important links which draws more attention. Based on this, the paper studies transport route of railway logistics and sets up its model of the optimal route combining with ant colony algorithm. It carries out simulation analysis of each parameter of ant colony algorithm and chooses the optimal region of corresponding parameter. By taking railway logistics in Hubei Province as an example, the optimal transport route plan and its schematic drawing are required through calculation, which provides the important guidance on railway logistics transportation under "the Belt and Road" strategy in China