The recent advances in spatial transcriptomics have brought unprecedented opportunities to understand the cellular heterogeneity in the spatial context. However, the current limitations of spatial technologies hamper the exploration of cellular localizations and interactions at single-cell level. Here, we present spatial transcriptomics deconvolution by topic modeling (STRIDE), a computational method to decompose cell-types from spatial mixtures by leveraging topic profiles trained from single-cell transcriptomics. STRIDE accurately estimated the cell-type proportions and showed balanced specificity and sensitivity compared to existing methods. We demonstrate STRIDE's utility by applying it to different spatial platforms and biological systems. Deconvolution by STRIDE not only mapped rare cell-types to spatial locations but also improved the identification of spatial localized genes and domains. Moreover, topics discovered by STRIDE were associated with cell-type-specific functions, and could be further used to integrate successive sections and reconstruct the three-dimensional architecture of tissues. Taken together, STRIDE is a versatile and extensible tool for integrated analysis of spatial and single-cell transcriptomics and is publicly available at https://github.com/DongqingSun96/STRIDE.

Aiming to advance a support problem for roadways used with the longwall coal mining method, the S1202 working face of the Ningtiaota Coal Mine is taken as the engineering background. The monitoring and analysis of bolt force, anchor cable force and surrounding rock deformation of two types of roadways within the whole advance pressure influence range are carried out in the present paper. Based on this, a numerical calculation model consistent with the field is established, and numerical comparison tests under different influencing factors are carried out. The rationality of the numerical test results is verified by using the field monitoring data. At the same time, quantitative evaluation indexes, such as characterization deformation, are established, and the deformation law of roadways surrounding rocks under different advance passive support forces is analyzed. The advance support mechanism of the roadway used with the longwall coal mining method is clarified. The test shows that under the condition of no advance passive support, the maximum characteristic deformation of surrounding rock in the haulage roadway and ventilation roadway is 7.1 cm and 10.1 cm, respectively. The above surrounding rock deformation still meets the requirements of on-site safety production. The research results can provide experimental support for the advance support parameters of the roadway used with the longwall coal mining method.