Introduction: Physical fitness is an essential factor affecting soccer in China, and its high-level results from the overlapping training effects of various factors on the soccer athlete. Objective: This study aims to analyze the relationship between practical fitness training and performance in college soccer players. Methods: College soccer players volunteered for a fitness training session. Mathematical statistics were used to analyze volunteers’ fitness and competition performance before and after training. The impact of soccer fitness training on the athletes’ physical quality was also considered. Results: After eight weeks of fitness training, improvement in the performance of college soccer players was noticed. There was a statistical difference in performance and physical quality after physical training (P<0.01). Conclusion: College soccer players improved performance after fitness training. Such training can improve the physical fitness of college soccer players. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.