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In order to determined the antibacterial property of Apocynum venetum. Three kinds of extractants (distilled water, 75% ethanol and absolute ethanol) were used to extract the leaf, stick and skin of Apocynum venetum, respectively. Flask and plate methods were used to evaluate the antibacterial property of these extracts on four kinds of classical microbiologies, Escherichia coli, Staphylococcus aureus, Saccharomyces cerevisiae and Aspergillus niger, respectively. The results showed that the antibacterial rate of extract by 75 % ethanol was better than extracts by water or absolute ethanol on Escherichia coli and skin contained more antibacterial substance (on Escherichia coli) than others. The antibacterial rate of extract by distill water was better than extracts by 75 % ethanol or absolute ethanol on Staphylococcus aureus and stick contained more antibacterial substance (on Staphylococcus aureus) than others. Some of extracts had very weak antibacterial rate on Saccharomyces cerevisiae and all extracts had not antibacterial effect on Aspergillus niger.