No abstract

Studies have indicated that dietary resveratrol (RES) improves the meat quality of broilers subjected to heat stress (HS), but the mechanism of action remains unclear. Therefore, the main purpose of this study was to investigate the effect of RES on meat quality, muscle antioxidant status, and its mechanism of action in broilers under HS. A total of 162 male AA broilers at 21 days old with similar weight were randomly assigned to 3 treatment groups with 6 replicates each. The control group (ambient temperature: 22 ± 1 °C) and HS group (ambient temperature: 33 ± 1 °C for 10 h a day from 8:00 to 18:00 and 22 ± 1 °C for the remaining time) were fed a basal diet and the HS + RES group was fed a basal diet with 400 mg/kg RES. The feeding was conducted for 21 continuous days. The results indicated that HS decreased final body weight (BW), average daily gain (ADG), average daily feed intake (ADFI), breast and leg muscle yield, a*24h, pH24h, the activities of catalase (CAT), glutathione S-transferase (GST) and glutathione peroxidase (GSH-Px), and mRNA levels of nuclear factor erythroid 2–related factor 2 (Nrf2), heme oxygenase-1 (HO-1), NADPH quinone oxidoreductase 1 (NQO1), and GSH-Px (p < 0.05). HS also increased b*45min, L*24h, drip loss, malondialdehyde (MDA) content, and kelch-like epichlorohydrin-associated protein 1 (Keap1) mRNA level (p < 0.05). Compared with the HS group, the HS + RES group exhibited a higher ADG, breast and leg muscle yield, a*24h, pH24h, activities of GST and GSH-Px, and mRNA levels of Nrf2, HO-1, and NQO1 but had lower drip loss and Keap1 mRNA level (p < 0.05). RES can improve meat quality and the muscle antioxidant ability of heat-stressed broilers by activating the Nrf2 signaling pathway.

This study investigated the effect of resveratrol on the immune and inflammatory responses and the mRNA levels of splenic toll-like receptor (TLR)-4 signaling pathway-related genes of broilers under heat stress (HS). One hundred and sixty-two birds were allocated to three groups, each with 6 replicates, for 21 continuous days. The three treatments were as follows: the control group (22 ± 1 °C), the HS (33 ± 1 °C for 10 h d -1 and 22 ± 1 °C for the remaining time) group and the HS + resveratrol (400 mg kg -1 ) group. At the end of the trial, one bird per replicate close to the average body weight (BW) was selected, exsanguinated, and slaughtered. Compared with the control group, the HS treatment decreased (p<0.05) final BW, average daily gain (ADG), average daily feed intake (ADFI), relative weight of bursa of Fabricius and spleen, serum immunoglobulin (Ig) Y, IgA and interleukin (IL)-10 contents, and splenic IL-10 mRNA level, while it increased (p<0.05) feed/gain, mRNA levels of splenic tumor necrosis factor-α (TNF-α), TLR-4, nuclear factor-kappa-B (NF-κB), IL-1β, and IL-6. Compared to the HS group, the HS+resveratrol group exhibited increased (p<0.05) final BW, ADG, relative weight of bursa of Fabricius and spleen, serum IgY, IgA and IL-10 contents, and splenic IL-10 mRNA level, while it exhibited lower (p<0.05) TNF-α, IL-1β and IL-6 contents in serum, and splenic TLR4, TNF-α, IL-1β, and NF-κB mRNA levels. In conclusion, resveratrol prevented a HS-impairment of the immune function of broilers by blocking the abnormal activation of the TLR4 signaling pathway.

Simple Summary Weaning stress causes retarded growth, gut disorder and dysfunction, severe diarrhea and higher mortality in weaned piglets. Antibiotic growth promoters (AGP) have been conventionally used to alleviate the negative effects of weaning stress. However, the long-term use of AGP leads to various adverse effects, such as antimicrobial resistance and food drug residues, and threatens public safety. Therefore, AGP should be replaced with residue-free, pollution-free and toxin-free alternatives because several countries and regions have banned the use of AGP in the feed industry. This study investigates the effects of an encapsulated organic acid and essential oil mixture (OAEO) on the growth performance, immuno-antioxidant capacity and intestinal health of weaned piglets. The results reveal that OAEO as an alternative to AGP improved the growth performance, immuno-antioxidant status and intestinal health of weaned piglets partly by activating the Nrf2 signaling pathway and suppressing the TLR4/NF-κB signaling pathway. Abstract This study investigates the effects of an encapsulated organic acid and essential oil mixture (OAEO) on the growth performance, immuno-antioxidant capacity and intestinal health of weaned piglets. In total, 120 weaned piglets (23 days of age; 6.96 ± 0.08 kg) were randomly allotted to four treatments (six replicates/group; five piglets/replicate): the control group (CON) was fed the basal diet (BD), the antibiotic growth promoters group (AGP) received the BD with 20 mg/kg colistin sulphate and 10 mg/kg bacitracin zinc, and OAEO1 and OAEO2 were fed the BD with 1000 mg/kg and 2000 mg/kg OAEO, respectively. The trial lasted 21 days and then one piglet per replicate was selected for sample collection. OAEO increased the average daily gain, spleen index, serum interleukin (IL)-10, immunoglobulin (Ig) G and IgA levels; serum superoxide dismutase and glutathione peroxidase (GPX) activities; and jejunal villus height (VH), VH/crypt depth, goblet cell number, and amylase and trypsin activities ( p < 0.05) compared with CON but reduced the diarrhea rate, serum tumor necrosis factor (TNF)-α, malondialdehyde (MDA), and D -lactic acid contents and diamine oxidase (DAO) activity ( p < 0.05). OAEO also increased the jejunal zonula occludens-1, occludin, claudin-1, mucin-2, nuclear factor erythroid 2-related factor 2 (Nrf2), GPX and IL-10 mRNA levels, GPX activity and IL-10 content ( p < 0.05) compared with CON but reduced jejunal MDA, IL-1β and TNF-α contents and Toll-like receptor (TLR) 4, nuclear factor (NF)-κB and TNF-α mRNA levels ( p < 0.05). In addition, AGP increased ADG, serum IgA level and GPX activity, jejunal trypsin activity and IL-10 content and mRNA level ( p < 0.05) compared with CON but reduced the serum TNF-α content and DAO activity and jejunal NF-κB mRNA level ( ...