Pituitary adenoma is one of the most common intracranial tumors, more and more studies have shown that long non-coding RNA (lncRNA) plays a very important role in pituitary adenoma. However, there are few reports on the function of lncRNA BBOX1-AS1 in pituitary adenomas, and further exploration is needed. The objective of this research is to figure out what function BBOX1-AS1 plays in pituitary adenoma and how it regulates it. The expression of the E2F1, miR-361-3p and BOX1-AS1 genes was measured using a quantitative real-time PCR method. The functional involvement of BBOX1-AS1 in pituitary adenoma was examined utilizing the Transwell assay, western blot assays and the cell counting kit-8. RNA immunoprecipitation and luciferase reporter assays revealed that miR-361-3p binds to E2F1 or BBOX1-AS1. In addition, in-vivo assays were carried out. The expression of BBOX1-AS1 in pituitary adenoma tissues and cells has been increased, according to our findings. Furthermore, it is also noted that downregulation of BBOX1-AS1causes the inhibition of pituitary adenoma cells which result in invasion, apoptosis and proliferation, as well as boosting tumor development in vivo. In addition, BBOX1-AS1 knockdown inhibited tumor development in vivo. We identify BBOX1-AS1 bind to miR-361-3p and to suppress its expression in a negative way. Moreover, miR-361-3p has been shown to bind with E2F1 and inhibit its expression. E2F1 also corrected miR-361-3p-mediated cell invasion, proliferation and apoptosis in BBOX1-AS1-dysregulated pituitary adenoma cells in rescue tests. BBOX1-AS1 increases pituitary adenoma malignant activity by sponging miR-361-3p to upregulate E2F1 expression, which may lead to a new path in pituitary adenoma therapeutic attempts.

Due to the lack of structural anti-floating, a certain part of the main structure has cracks, and the structure needs to be reinforced by buoyancy to make the project meet the requirements.After research, the Negative second floor of the original structure is reinforced with add the anti-floating anchors, apply pressure grouting for cracks in the plate, Paste carbon fiber cloth for the bottom of the plate and the damaged shear wall, externally-paste profile steel for the damaged column, and paste Carbon fiber sheet attached to damaged beam.the calculated of shear bearing and flexural capacity show that the board meets the requirements.

Spherical roller bearings are important parts of high-frequency crushers. Using the finite element analysis software to carry on the transient dynamics analysis to the bearing obtains the bearing deformation, the pressure and so on characteristis. Then, by contrasting and analysising, it is found that under pure radial load, radial load and axial load mixed force, influence of cage thickness on bearing performance. The results show that the thickness of the cage has little effect on the deformation of the bearing under pure radial load. Under the combined action of the radial load and the axial load, the radial deformation of the bearing decreases compared with the pure radial load. The deformation of the cage under the mixed load is increased than that under the radial load.

In this paper, the basic principle, secondary software development and practical application of viscous damper vibration reduction technology are studied. The viscous damping coefficient velocity function equivalence method is used, and the secondary development is carried out in the finite element software ANSYS through program compilation, and the operation interface of the velocity function equivalence method is established. On this basis, the complex vibration problems of an existing building are studied, and the arrangement and installation of viscous dampers are guided on site.Through comparative analysis, it is concluded that the UIDL program and APDL macro command stream in the finite element software ANSYS can be used to establish the operation interface of the speed function equivalent method, which makes the method have better applicability and operability, improve the work efficiency and facilitate the popularization and application. By adopting the nonlinear viscous damper damping technology, before the structure and equipment produce strong vibration, the damper first enters the energy dissipation state, produces large damping, greatly consumes vibration energy, and rapidly attenuates the dynamic response of the structure and equipment to ensure the safety and normal use of the structure and equipment.The field dynamic test results and the finite element analysis results both show that the viscous damper vibration reduction technology based on the velocity function equivalent method has a significant vibration reduction effect, and is an effective means to solve the existing building vibration problems.