Biomineralization is a known natural phenomenon associated with a wide range of bacterial species. Bacterial-induced calcium carbonate precipitation by marine isolates was investigated in this study. Three genera of ureolytic bacteria, Sporosarcina sp., Bacillus sp. and Brevundimonas sp. were observed to precipitate calcium carbonate minerals. Of these species, Sporosarcina sp. dominated the cultured isolates. B. lentus CP28 generated higher urease activity and facilitated more efficient precipitation of calcium carbonate at 3.24 ± 0.25 × 10−4 mg/cell. X-ray diffraction indicated that the dominant calcium carbonate phase was calcite. Scanning electron microscopy showed that morphologies of the minerals were dominated by cubic, rhombic and polygonal plate-like crystals. The dynamic process of microbial calcium carbonate precipitation revealed that B. lentus CP28 precipitated calcite crystals through the enzymatic hydrolysis of urea, and that when ammonium ion concentrations reached 746 mM and the pH reached 9.6, that favored calcite precipitation at a higher level of 96 mg/L. The results of this research provide evidence that a variety of marine bacteria can induce calcium carbonate precipitation, and may influence the marine carbonate cycle in natural environments.

Microbiologically induced deterioration (MID) causes corrosion of concrete by producing acids (including organic and inorganic acids) that degrade concrete components and thus compromise the integrity of sewer pipelines and other structures, creating significant problems worldwide. Understanding of the fundamental corrosion process and the causal agents will help us develop an appropriate strategy to minimize the costs in repairs. This review presents how microorganisms induce the deterioration of concrete, including the organisms involved and their colonization and succession on concrete, the microbial deterioration mechanism, the approaches of studying MID and safeguards against concrete biodeterioration. In addition, the uninvestigated research area of MID is also proposed.

The beach‐bar reservoir play has become an important exploration target within the Bohai Bay Basin, especially in the Boxing Sag within the Dongying Depression, where a large‐scale lacustrine beach‐bar oil pool has been discovered recently. The sedimentary characteristics, distribution patterns and formation mechanisms of beach‐bar sand bodies in the upper fourth member of the Eocene Shahejie Formation (Es4s) in the Boxing Sag were studied in detail based on seismic, well log data and core data. The Es4s in the Boxing Sag is composed of a third‐order sequence consisting of three systems tracts, i.e. a lowstand systems tract, a transgressive systems tract and a highstand systems tract. Beach‐bar sand bodies were deposited widely in the basin during the lowstand systems tract period. The sandy beach‐bars are characterized by siltstones, fine‐grained silty sandstones interbedded with thin mudstone units. The presence of well‐developed sedimentary structures, such as swash bedding, parting lineation, parallel bedding, ellipsoidal mud clasts, ripples, terrestrial plant debris and vertical burrows, suggests that beach‐bars were deposited in a relatively shallow water environment under the influence of strong hydrodynamics. Laterally, the sandy beach facies occurred as a more continuous sheet‐like body around the sandy bar in most parts of the sag. Stratigraphically, beach‐bars were distributed mainly in the lowstand systems tract and they were less well‐developed in the transgressive systems tract and highstand systems tract. Several factors were probably responsible for the occurrence of the large‐scale beach‐bars during the lowstand systems tract period, including: (i) a gentle palaeoslope and relatively weak structural activities; (ii) a shallow‐water condition with a strong hydrodynamic environment; (iii) high‐frequency oscillations of the lake level; and (iv) an abundant terrigenous clastic feeding system with multiple‐point and linear sediment sources.

A sediment core collected from coastal zone near the Qiao Island in the Pearl River Estuary was analyzed for total metal concentrations, chemical partitioning, and physico-chemical properties. Three vertical distribution patterns of the heavy metals in the sediment core were identified, respectively. The dominant binding phases for Cu, Pb, Cr, and Zn were the residual and Fe/Mn oxides fractions. Cd in all sediments was mainly associated with exchangeable fraction. Influences of total organic carbon content and cation exchange capacity on the total concentrations and fractions of almost all the metals were not evident, whereas sand content might play an important role in the distributions of residual phases of Cr, Cu, Pb, and Zn. In addition, sediment pH had also an important influence on the Fe/Mn oxides, organic/sulfide and residual fractions of Cr, Cu, and Zn. Contamination assessment on the heavy metals in the sediment core adopting Index of Geoaccumulation showed that Cr, V, Be, Se, Sn, and Tl were unpolluted, while Cu, Ni, Pb, Zn, Cd, and Co were polluted in different degrees throughout the core. It was remarkable that the various pollution levels of the metals from moderate (for Cu, Pb, and Zn) to strong (for Cd) were observed in the top 45 cm of the profiles. The relative decrease of the residual fraction in the upper 45 cm of the core is striking, especially for Zn and Cu, and, also for Pb, and Cr. The change in fraction distribution in the upper 45 cm, which is very much contrasting to the one at larger depths, confirms that the residual fraction is related to the natural origin of these metals, whereas in the upper part, the non-residual fractions (mainly the Fe/Mn oxides fraction) are increased due to pollution in the last decade. The possible sources for Cu, Pb, Zn, and Cd contaminations were attributed to the increasing municipal and industrial wastewater discharges, agricultural runoff, atmospheric inputs, and runoff from upstream mining or smelting activities, which may be associated with an accelerating growth of economy in the Pearl River Delta region in the past decade.

No abstract