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Fruit rot is one of the most important factors affecting the postharvest quality and shelf life of Lanzhou Lily fruits. The aims of this study were to characterize different isolates from Lanzhou Lily using morphological and molecular approaches and to test their pathogenicity in Lanzhou Lily fruit. Different isolates were collected from decayed Lanzhou Lily fruits in Lanzhou in Gansu Province in China during 2016 and 2017. In total, four isolates were obtained and identified based on their DNA sequences of the 16S rDNA and 26S rDNA genes in combination with the morphological characteristics of the cultures and sporulation. Of the four isolates, one was identified as Bacillus safensis, one was Stenotrophomonas maltophilia and the other two isolates were Metschnikowia pulcherrima. The pathogenicity tests showed that all four isolates were pathogenic in Lanzhou Lily fruit. Previously Fusarium tricinctum has been reported to be the primary cause of fruit rot in Lanzhou Lily throughout the world. Our report is the first to show results that indicate that B. safensis, S. maltophilia and M. pulcherrima are pathogenic in Lanzhou Lily in China. This is the first report on the bacteria and yeast that infect Lanzhou Lily fruits.

The growth of Phytophthora capsica, Rhizoctonia solani, Fusarium graminearum, Fusarium oxysporum and Botrytis cinerea were all inhibited by the fermentation supernatant of Bacillus licheniformis TG116, a biocontrol strain isolated from Typhonium giganteum Engl previously with broad-spectrum resistance to plant pathogens. The fermentation supernatant of the TG116 has a great stability on temperature and UV, and shows the biological activity of serine protease and cellulase. The antifungal protease produced by Bacillus licheniformis TG116 was puri ed to homogeneity by ammonium sulfate precipitation, DEAE Sepharose Fast Flow column chromatography and Sephadex G-50 column chromatography. When Colletotrichum capsici was used as an indicator strain, the protease exhibited great antifungal activity at a pH range from 5.0 to 11.0 and temperature at no higher than 60°C. Gene ampli cation veri ed the presence of a gene fragment of serine protease in the strain TG116. The antimicrobial substance isolated from the fermentation broth of TG116 is a serine protease component. The serine protease isolated and puri ed from B. licheniformis TG116 has a great antifungal activity.

No abstract

The growth of Phytophthora capsica, Rhizoctonia solani, Fusarium graminearum, Fusarium oxysporum and Botrytis cinerea were all inhibited by the fermentation supernatant of Bacillus licheniformis TG116, a biocontrol strain isolated from Typhonium giganteum Engl previously with broad-spectrum resistance to plant pathogens. The fermentation supernatant of the TG116 has a great stability on temperature and UV, and shows the biological activity of serine protease and cellulase. The antifungal protease produced by Bacillus licheniformis TG116 was purified to homogeneity by ammonium sulfate precipitation, DEAE Sepharose Fast Flow column chromatography and Sephadex G-50 column chromatography. When Colletotrichum capsici was used as an indicator strain, the protease exhibited great antifungal activity at a pH range from 5.0 to 11.0 and temperature at no higher than 60°C. Gene amplification verified the presence of a gene fragment of serine protease in the strain TG116. The antimicrobial substance isolated from the fermentation broth of TG116 is a serine protease component. The serine protease isolated and purified from B. licheniformis TG116 has a great antifungal activity.