Tea polyphenol (TPs) oxidation caused by polyphenol oxidase (PPO) in manufacturing is responsible for the sensory characteristics and health function of fermented tea, therefore, this subject is rich in scientific and commercial interests. In this work, an in vitro catalysis of TPs in liquid nitrogen grinding of sun-dried green tea leaves by PPO was developed, and the changes in metabolites were analyzed by metabolomics. A total of 441 metabolites were identified in the catalyzed tea powder and control check samples, which were classified into 11 classes, including flavonoids (125 metabolites), phenolic acids (67 metabolites), and lipids (55 metabolites). The relative levels of 28 metabolites after catalysis were decreased significantly (variable importance in projection (VIP) > 1.0, p < 0.05, and fold change (FC) < 0.5)), while the relative levels of 45 metabolites, including theaflavin, theaflavin-3′-gallate, theaflavin-3-gallate, and theaflavin 3,3′-digallate were increased significantly (VIP > 1.0, p < 0.05, and FC > 2). The increase in theaflavins was associated with the polymerization of catechins catalyzed by PPO. This work provided an in vitro method for the study of the catalysis of enzymes in tea leaves.

Alcoholic fatty liver disease (AFLD) is caused by long-term heavy alcohol consumption; therefore, useful and practical methods for the prevention of AFLD are urgently needed. The edible flower of Dendrobium officinale contains diverse flavonoids, and has shown antioxidant activity as well as antihypertensive and anti-inflammatory effects. In this study, an AFLD model was established, the protective effect of D. officinale flower (DOF) ethanol extract on AFLD was evaluated, and its mechanisms were investigated by analyzing gut microbiota and short-chain fatty acids (SCFAs). DOF extract (DOFE) supplementation promoted alcohol metabolism, restored hepatic antioxidant capacity, alleviated oxidative stress, reduced inflammatory factor levels, and inhibited dyslipidemia induced by alcohol intake in chronic alcohol-exposed mice, especially in the high DOFE group. Moreover, DOFE supplementation increased the diversity, structure, and composition of the gut microbiota in mice, restored some of the abnormal SCFA levels caused by AFLD, and helped restore intestinal function. DOFE supplementation significantly increased the relative abundance of Akkermansia, suggesting that Akkermansia may be a potential target of the protective effect of DOFE. Therefore, DOFE supplementation to improve the composition of the gut microbiota may be an effective therapeutic strategy for the prevention of AFLD.

Tea tree [Camellia sinensis var. sinensis or assamica (L.) O. Kuntze], an important crop worldwide, is usually pruned to heights of 70 to 80 cm, forming pruned tea tree (PTT) plantations. Currently, PTTs are transformed into unpruned tea tree (UPTT) plantations in Yunnan, China. This has improved the quality of tea products, but the underlying reasons have not been evaluated scientifically. Here, 12 samples of sun-dried green teas were manufactured using fresh leaves from an UPTT and the corresponding PTT. Using sensory evaluation, it was found that the change reduced the bitterness and astringency, while increasing sweetness and umami. Using high performance liquid chromatography detection showed that the contents of free amino acids (theanine, histidine, isoleucine and phenylalanine) and catechin gallate increased significantly (P &lt; 0.05), whereas the content of alanine decreased significantly (P &lt; 0.05). A liquid chromatography–mass spectrometry-based metabolomics analysis showed that the transformation to UPTT significantly decreased the relative levels of the majority of flavonols and tannins (P &lt; 0.05), as well as γ-aminobutyric acid, caffeine and catechin (epigallocatechin, catechin, epigallocatechin gallate, gallocatechin gallate), while it significantly increased the relative contents of catechins (gallocatechin, epicatechin, epicatechin gallate and catechin gallate), phenolic acids and some amino acids (serine, oxidized glutathione, histidine, aspartic acid, glutamine, lysine, tryptophan, tyramine, pipecolic acid, and theanine) (P &lt; 0.05). In summary, after transforming to UPTT, levels of amino acids, such as theanine increased significantly (P &lt; 0.05), which enhanced the umami and sweetness of tea infusions, while the flavonoids (such as kaempferol, myricetin and glycosylated quercetin), and caffeine contents decreased significantly (P &lt; 0.05), resulting in a reduction in the bitterness and astringency of tea infusions and an increase in tea quality.

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In order to broaden the application field of proanthocyanidins (PCs) from Zijuan tea, it is necessary to establish an environmental and economic extraction method of PCs from Zijuan tea. Based on the single‐factor experiment, Box‐Behnken response surface methodology was used to study the effects of solid–liquid ratio, the concentration of citric acid solution, ultrasonic power, ultrasonic time, and ultrasonic temperature on the yield of PCs from Zijuan tea. The results showed that the optimum extraction conditions were as follows: 1:35.3 (m/V) solid–liquid ratio, 0.08% citric acid solution, 233.0 W ultrasonic power, 64.0°C ultrasonic temperature, and 48.6 min ultrasonic time. Under these conditions, the yield of PCs was 5.18 ± 0.13 mg/g. Meanwhile, the half inhibitory concentration (IC50) of PCs on 1,1‐diphenyl‐2‐picrylhydrazyl radical (DPPH) and hydroxyl radical (·OH) were 0.5417 and 0.4825 mg/mL, respectively; antimicrobial activity of PCs on several strains tested showed antimicrobial effect as follows: Bacillus subtilis >Staphylococcus aureus >Escherichia coli >Saccharomyces cerevisiae. Therefore, the results provided a theoretical basis for the application of PCs in food development. Novelty impact statement The extraction conditions significantly affected the yield of proanthocyanidins (PCs) from Zijuan tea. While Zijuan tea extracted with 0.08% the citric acid solution (1:35.3 [m/V] solid–liquid ratio) and ultrasonic instrument (233.0 W ultrasonic power, 64.0°C ultrasonic temperature, and 48.6 min ultrasonic time) could be used as a potential food additive, exhibiting a promising antioxidant potential for food product applications.