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Major royal jelly protein 1 (MRJP1) is the most abundant member of the major royal jelly protein (MRJP) family of honeybee. Mature MRJP1 cDNA of the Chinese honeybee (Apis cerana cerana MRJP1, or AccMRJP1) was expressed in Pichia pastoris. SDS-PAGE showed that recombinant AccMRJP1 was identical in molecular weight to the glycosylated AmMRJP1 from the Western honeybee (Apis mellifera). Western blots probed with anti-AccMRJP1 antibody demonstrated that recombinant AccMRJP1 and soluble protein of the Western honeybee RJ (AmSPRJ) contained immunoreactive MRJP1. The 57 kDa protein in AmSPRJ contained an N-terminal amino sequence of N-I-L-R-G-E, which is identical to that previously characterized in AmMRJP1. The molecular weight of recombinant AccMRJP1 was decreased from 57 to 48 kDa after deglycosylation, indicating that AccMRJP1 was glycosylated. The recombinant AccMRJP1 significantly stimulated Tn-5B-4 cell growth, similar to AmSPRJ and fetal bovine serum, and affected cell shape and adhesion to the substrate.

Blockchain technology is the most cutting-edge technology in the field of financial technology, which has attracted extensive attention from governments, financial institutions and investors of various countries. Blockchain and finance, as an interdisciplinary, cross-technology and cross-field topic, has certain limitations in both theory and application. Based on the bibliometrics data of Web of Science, this paper conducts data mining on 759 papers related to blockchain technology in the financial field by means of co-word analysis, bi-clustering algorithm and strategic coordinate analysis, so as to explore hot topics in this field and predict the future development trend. The experimental results found ten research topics in the field of blockchain combined with finance, including blockchain crowdfunding, Fintech, encryption currency, consensus mechanism, the Internet of Things, digital financial, medical insurance, supply chain finance, intelligent contract and financial innovation. Among them, blockchain crowdfunding, Fintech, encryption currency and supply chain finance are the key research directions in this research field. Finally, this paper also analyzes the opportunities and risks of blockchain development in the financial field and puts forward targeted suggestions for the government and financial institutions.