Formation constants of 3-hydroxy-2-naphthoic acid with β-cyclodextrin were determined in aqueous solutions without and with almost all the buffers usually used by steady-state fluorescence Benesi-Hildebrand method. The results show that buffers have influence on the formation constants and we suggest that buffers should no be used in aqueous solutions of cyclodextrins unless the experimental error is unacceptable.
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