In this paper, a symmetrically coated damping structure for entangled metallic wire materials (EMWM) of pipelines was designed to reduce the vibration of high temperature (300 °C) pipeline. A series of energy dissipation tests were carried out on the symmetrically coated damping structure at 20–300 °C. Based on the energy dissipation test results, the hysteresis loop was drawn. The effects of temperature, vibration amplitude, frequency, and density of EMWM on the energy dissipation characteristics of coated damping structures were investigated. A nonlinear energy dissipation model of the symmetrically coated damping structure with temperature parameters was established through the accurate decomposition of the hysteresis loop. The parameters of the nonlinear model were identified by the least square method. The energy dissipation test results show that the symmetrically coated damping structure for EMWM of pipelines had excellent and stable damping properties, and the established model could well describe the changing law of the restoring force and displacement of the symmetrically coated damping structure with amplitude, frequency, density, and ambient temperature. It is possible to reduce the vibration of pipelines in a wider temperature range by replacing different metal wires.

Background Colorectal cancer (CRC) is one of the leading causes of cancer-related death worldwide. Single-cell transcriptome sequencing (scRNA-seq) can provide accurate gene expression data for individual cells. In this study, a new prognostic model was constructed by scRNA-seq and bulk transcriptome sequencing (bulk RNA-seq) data of CRC samples to develop a new understanding of CRC. Methods CRC scRNA-seq data were downloaded from the GSE161277 database, and CRC bulk RNA-seq data were downloaded from the TCGA and GSE17537 databases. The cells were clustered by the FindNeighbors and FindClusters functions in scRNA-seq data. CIBERSORTx was applied to detect the abundance of cell clusters in the bulk RNA-seq expression matrix. WGCNA was performed with the expression profiles to construct the gene coexpression networks of TCGA-CRC. Next, we used a tenfold cross test to construct the model and a nomogram to assess the independence of the model for clinical application. Finally, we examined the expression of the unreported model genes by qPCR and immunohistochemistry. A clone formation assay and orthotopic colorectal tumour model were applied to detect the regulatory roles of unreported model genes. Results A total of 43,851 cells were included after quality control, and 20 cell clusters were classified by the FindCluster () function. We found that the abundances of C1, C2, C4, C5, C15, C16 and C19 were high and the abundances of C7, C10, C11, C13, C14 and C17 were low in CRC tumour tissues. Meanwhile, the results of survival analysis showed that high abundances of C4, C11 and C13 and low abundances of C5 and C14 were associated with better survival. The WGCNA results showed that the red module was most related to the tumour and the C14 cluster, which contains 615 genes. Lasso Cox regression analysis revealed 8 genes (PBXIP1, MPMZ, SCARA3, INA, ILK, MPP2, L1CAM and FLNA), which were chosen to construct a risk model. In the model, the risk score features had the greatest impact on survival prediction, indicating that the 8-gene risk model can better predict prognosis. qPCR and immunohistochemistry analysis showed that the expression levels of MPZ, SCARA3, MPP2 and PBXIP1 were high in CRC tissues. The functional experiment results indicated that MPZ, SCARA3, MPP2 and PBXIP1 could promote the colony formation ability of CRC cells in vitro and tumorigenicity in vivo. Conclusions We constructed a risk model to predict the prognosis of CRC patients based on scRNA-seq and bulk RNA-seq data, which could be used for clinical application. We also identified 4 previously unreported model genes (MPZ, SCARA3, MPP2 and PBXIP1) as novel oncogenes in CRC. These results suggest that this model could potentially be used to evaluate the prognostic risk and provide potential therapeutic targets for CRC patients.

Entangled metallic wire material (EMWM) is a kind of porous damping material. To promote the engineering application of EMWM, it is necessary to establish the constitutive model of EMWM to estimate its mechanical properties. In this paper, a series of quasi-static compression experiments for plate-like EMWM specimens made of austenitic stainless steel wire (06Cr19Ni10) with different densities were carried out in the temperature range of 20–500 °C. It was found that the stiffness of the plate-like EMWM would increase with the increases in the ambient temperature. The non-linear characteristics of the force–displacement curve of the plate-like EMWM would be weakened. Taking the spatial structural characteristics of the plate-like EMWM and the influence of the thermal expansion of the structure into account, a new constitutive model for plate-like EMWM was presented by the combination of the Johnson–Cook model and the Sherwood–Frost constitutive framework model. The accuracy of the model was verified by the experimental data under different temperatures. The results show that the calculated results of the model are consistent with the experimental results. This model can provide an effective theoretical basis for predicting the mechanical properties of plate-like EMWM and guiding its design.

When the submarine is sailing at full speed, the power cabin has an abnormally high temperature. However, in the previous research on the vibration reduction design of the foundation, the influence of high temperature on the vibration characteristics of the foundation is not taken into account. In this paper, a new composite foundation with entangled metallic wire material (EMWM) is presented to reduce the vibration of the foundation. The energy transfer path of the foundation was obtained by the power flow method, and then the layout of EMWM was determined. The optimization of the constraining layer was carried out by modal analysis. The damping performance of the composite foundation with EMWM was validated by the thermal-vibration joint test. The results show that, at room temperature, the composite foundation has remarkable vibration reduction efficiency in the middle and high-frequency bands. The maximum insertion loss can reach 15.37 dB. The insertion loss varies with the location of the excitation point. As the temperature rises to 300°C, the insertion loss in the low-frequency band was improved, and the insertion loss is not influenced by the excitation position.