A Rhizopus strain producing esterifying enzyme was isolated from Luzhou-flavor DaQu. It was preliminary identified as Rhizopus arrhizus. Through the research of the culture conditions of the strain producing esterifying enzyme, it was found that the enzyme activity could reach 7.91U/mL as 2% corn meal used as C source, 6% peptone used as N source, initial pH value was 6.0, and 72h culture with 150 r/min agitation at 30°C.

The metabolies characterization of mold producing esterifying synthetase of different culture conditions were studied. A mold strain producing esterifying enzyme was isolated from Luzhou-flavor DaQu and it was identified by Biolog Microbes automated identification System. Culture conditions such as culture temperature, fermentation time and carbon source were changed. The fermentatiom broth was extracted by ethanol and analyzed by GC-MS. The results showed that the strain was Aspergillus flavus. When the culture temperature increased, the molecular weight of its metabolites increased, the synthesis of high alcohol increased too, and their carbon chain lengthened gradually. When incubation time was prolonged, the varying law of the synthesis of its metabolites was alcohols-acid-ester, the metabolites with longer carbon chain produced earlier than the metabolites with shorter carbon chain. When the carbon source were starch, sucrose and bran, n-butyl alcohol and isoamyl alcohol were the common metabolites. When the carbon source was sucrose, most of the metabolites were alcohol and acid with shorter carbon chain. When the carbon source was starch, diethyl succinate was produced. When the carbon source was bran, the metabolites were less.