Arginine methylation is an important posttranslational modification catalyzed by protein arginine methyltransferases (PRMTs). However, the role of PRMTs in colorectal cancer (CRC) progression is not well understood. Here we report that non-POU domain-containing octamer-binding protein (NONO) is overexpressed in CRC tissue and is a potential marker for poor prognosis in CRC patients. NONO silencing resulted in decreased proliferation, migration, and invasion of CRC cells, whereas overexpression had the opposite effect. In a xenograft model, tumors derived from NONO-deficient CRC cells were smaller than those derived from wild-type (WT) cells, and PRMT1 inhibition blocked CRC xenograft progression. A mass spectrometry analysis indicated that NONO is a substrate of PRMT1. R251 of NONO was asymmetrically dimethylated by PRMT1 in vitro and in vivo. Compared to NONO WT cells, NONO R251K mutant-expressing CRC cells showed reduced proliferation, migration, and invasion, and PRMT1 knockdown or pharmacological inhibition abrogated the malignant phenotype associated with NONO asymmetric dimethylation in both KRAS WT and mutant CRC cells. Compared to adjacent normal tissue, PRMT1 was highly expressed in the CRC zone in clinical specimens, which was correlated with poor overall survival in patients with locally advanced CRC. These results demonstrate that PRMT1-mediated methylation of NONO at R251 promotes CRC growth and metastasis, and suggest that PRMT1 inhibition may be an effective therapeutic strategy for CRC treatment regardless of KRAS mutation status.

This paper analyzes the mechanism of the GNOME and ADIOS models. On this basis, GNOME and ADIOS model are applied to the simulation of the oil drift and the weathering process of the early oil spill in the Gulf of Mexico respectively by correctly adding the Gulf of geographical information and environmental information. The simulated oil spill trajectories agree well with remote sensing monitoring results and the simulation results of ADIOS are in line with the oil spill weathering study conclusions. This paper also analyzes the reasons of simulation mistakes and the shortcomings of the model itself so as to figure out the direction for the future study.

With the acceleration of urbanization, Combined Sewer Overflows(CSOS) has become one of the important sources of polluted urban water. This paper overviewed the domestic and foreign CSOs current situation, and analysed the problems and deficiencies in the regulatory of CSOs in China. According to the characteristics of CSOs, we suggested that the regulatory of CSOs focus on the source, pipes and ends of the drainage system. The conclusion would be evidence for improving the combined sewerage system in China.

Pore structure of soft-slicing coal (SSC) is analyzed with physical adsorption method. Several experiment schemes are designed to carry out the tests of gas seepage characteristics of soft-slicing coal at the different confining pressures, gas pressures and axial pressures, with the self-developed gas seepage triaxial pressure experimental system. The typical coal from TianFu coal mine is especially sampled with different designed surface area parameters. The experiment results demonstrate the relations between the seepage velocity, the effective axial feed, and gas pressure. Under the conditions of the constant axial and confining pressure, the seepage velocity increases in an exponent way with gas pressure increasing, the permeability coefficient decreases in negative exponent, and this seepage process shows capacity fractal properties.

Based on the analysis of heat and mass transfer and an empirical correlation, a new desalination unit which utilizes solar or waste energy has been developed and tested. In this desalination, a considerable fraction of the latent and sensible heat was successfully recycled and utilized for enhancing falling evaporation-condensation and preheating seawater via remaining heat. The performance of the unit was tested through experiments using an electric heater as heat source. The results indicate that the unit has excellent transient-state performance for effective heat and mass transfer. As a result of the unit operating with a solar system under practical weather conditions, the yield was about five times greater than that of a conventional single solar basin still with the same thermal energy input. The main factors which influence the freshwater yield were researched and analyzed. The reasonable ranges of some parameters were presented.