The purpose of this study was to investigate the association between an inhibitor of apoptosis (survivin) and vascular endothelial growth factor (VEGF) expression in patients with acute lymphoblastic leukemia (ALL) prior to and following chemotherapy. Reverse transcription (RT)-PCR and western blotting were employed to analyze survivin and VEGF mRNA and protein expression. Moreover, the concentrations of survivin and VEGF were determined by enzyme-linked immunosorbent assay (ELISA). Our data revealed that survivin and VEGF were overexpressed in patients with ALL prior to treatment, while survivin levels were significantly decreased following treatment. In addition, there was a positive correlation between survivin and VEGF concentrations in plasma. In conclusion, analysis of survivin and VEGF expression levels may improve the clinical diagnosis and treatment of ALL.

BackgroundAcute hemorrhagic necrotizing pancreatitis (AHNP) is a severe acute inflammatory of pancreas that can lead to extrapancreatic organ disfunction. The lung and intestine is the most common involved organs, and abdominal lymphatic flow may contribute to AHNP- associated organ injury. In this study, we investigated the impact of thoracic duct ligation and drainage on lung and intestine injury in rats with AHNP.MethodsThirty-two male Wistar rats were randomly divided into 4 groups: sham operation group, AHNP group, AHNP + ligation group and AHNP + drainage group. Rat AHNP model was induced by retrograde injection of 3.5% sodium deoxycholate into the biliopancreatic duct, and the sham operation group was injected only with saline. In AHNP + ligation group and AHNP + drainage group, thoracic duct was ligated or drainaged before model induction. At 6 h after model induction, the bronchoalveolar lavage fluid (BALF) were collected for determination of tumor necrosis factor-alpha (TNF-alpha), and the tissues of lung, intestine and pancrease were harvested individually for pathohistological evaluation and the myeloperoxidase (MPO) activity determination. In addition, the activity of serum amylase and diamine oxidase (DAO) was determined in each group.ResultsThe pathohistological damage and MPO of lung, intestine and pancrease, TNF-alpha of BALF, serum amylase and DAO were all increased in AHNP group compared to those in sham operation group (P < 0.05). In AHNP + ligation group, the pathohistological damage and MPO of lung and TNF-alpha of BALF were reduced, but the pathohistological damage and MPO of intestine and pancrease were increased compared with AHNP group (P < 0.05), however the activity of serum amylase and DAO was no changed. In AHNP + drainage group, the pathohistological damage and MPO of lung, intestine and pancrease, TNF-alpha of BALF, serum amylase and DAO were all reduced compared to those in AHNP group (P < 0.05).ConclusionsOur finding suggest that thoracic duct ligation can reduce neutrophil infiltration and TNF-alpha release and then attenuates lung injury in rats with AHNP, but aggravates the injury of intestine and pancrease. While thoracic duct drainage attenuates the injury of lung, intestine and pancreaseat the same time in rats with AHNP.