Esophageal squamous cell carcinoma (ESCC) is one of the human malignancies worldwide, but the mechanism of ESCC development is still unclear. Chloroquine has the anti-tumor function by the inhibition of autophagy and thereby contributing to apoptosis. In our study, we analyzed the RNA-seq data of Chloroquine-treated ESCC cells and identified the transcriptional landscapes. We then used the gene enrichment methods such as KEGG and GO to further analyze the potential signaling pathways. In addition, we constructed the PPI network and Reactome map to further identified the biological processes. We identified the top two signaling pathways that were involved in the chloroquine-treated ESCC: Cell cycle and Glycerophospholipid metabolism. We identified the top ten interactive genes including ATM, CCNB1, FN1, CCT6A, VEGFA, PA2G4, CCT2, CDKN1A, BRIX1, and CDC20. Our study may provide new insights into the mechanisms for the Chloroquine-treated ESCC cells.

Lactate dehydrogenase (LDHA) is a metabolism-related enzyme that is involved in the progression of several cancers such as pancreatic ductal adenocarcinoma (PDAC). However, the mechanism of LDHA regulates the PDAC is largely unknown. In this study, we analyzed the RNA-seq data of LDHA inhibitor-treated PDAC cells and identified the transcriptional landscapes of these cells. We used the gene enrichment assays including KEGG and GO to further analyzed the potential signaling pathways. In addition, we constructed the PPI network and Reactome map to further identified the biological processes. We found the TNF signaling pathway and Cellular senescence are the major signaling pathways that involve the LDHA inhibitor-treated PDAC cells. We identified the top ten interactive genes including FN1, CCND1, HDAC1, NFKBIA, PPARG, HSPA5, AURKB, CXCR4, RUNX2, and IRF1. Our study may provide novel mechanisms for the treatment of PDAC.

LAMP5 is a lysosome-related protein, which showed increased expression levels in MLL-r leukemia. Recent findings showed that LAMP5 is involved in MLL-fusion protein but the specific mechanism is still unclear. In our study, we aim to identify the transcriptional landscapes of the LAMP5-shRNA-treated MLL-r leukemia cells. We used the gene enrichment analyses such as KEGG and GO to further analyze the potential signaling pathways. Moreover, we constructed the PPI network and Reactome map to further identify the biological processes. We found that Lysosome and Cell Cycle are the major signaling pathways involved in the LAMP5-shRNA treated MLL-r leukemia cells. We identified the top ten interactive genes including CDK1, TOP2A, PLK1, CCNA2, ASPM, BUB1, BUB1B, CCNB1, AURKA, and KIF11. Our study may provide novel mechanisms for the treatment of MLL-r leukemia by inhibiting LAMP5.