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Postmenopausal osteoporosis is associated with bone formation inhibition mediated by the impaired osteogenic differentiation potential of bone marrow mesenchymal stem cells (BMSCs). However, identifying and confirming the essential genes in the osteogenic differentiation of BMSCs and osteoporosis remain challenging. The study aimed at revealing the key gene that regulated osteogenic differentiation of BMSCs and led to osteoporosis, thus exploring its therapeutic effect in osteoporosis. In the present study, six essential genes related to the osteogenic differentiation of BMSCs and osteoporosis were identified, namely, fibrillin 2 (Fbn2), leucine‐rich repeat‐containing 17 (Lrrc17), heat shock protein b7 (Hspb7), high mobility group AT‐hook 1 (Hmga1), nexilin F‐actin‐binding protein (Nexn), and endothelial cell‐specific molecule 1 (Esm1). Furthermore, the in vivo and in vitro experiments showed that Hmga1 expression was increased during the osteogenic differentiation of rat BMSCs, while Hmga1 expression was decreased in the bone tissue of ovariectomized (OVX) rats. Moreover, the expression of osteogenic differentiation‐related genes, the activity of alkaline phosphatase (ALP), and the number of mineralized nodules were increased after Hmga1 overexpression, which was partially reversed by a Wnt signaling inhibitor (DKK1). In addition, after injecting Hmga1‐overexpressing lentivirus into the bone marrow cavity of OVX rats, the bone loss, and osteogenic differentiation inhibition of BMSCs in OVX rats were partially reversed, while osteoclast differentiation promotion of BMSCs in OVX rats was unaffected. Taken together, the present study confirms that Hmga1 prevents OVX‐induced bone loss by the Wnt signaling pathway and reveals that Hmga1 is a potential gene therapeutic target for postmenopausal osteoporosis.

Currently it is an important trend that outsourcing software has become the world's software industry development. At present many domestic software companies try to do outsourcing. But how to effectively control the outsourcing software requirements change has become a key point of outsourcing software success. Considering changing characteristics of software requirements, this paper seeks an effective software requirements change control method. How to effectively control changing software requirment is the key to achieve the software quality. This paper proposes dynamic quality function deployment (DQFD) method and leads DQFD in the process of software development. Combined with a cross-cultural communication theory, this paper discusses realizaing the outsourcing software requirement change control by DQFD and cross-cultural communication.

Requirements change has become a great challenge in software development, in order to control requirements change more effectively, it's necessary to extend or improve the traditional Quality Function Deployment (QFD) method. Thus, on the basis of model of SECI a new methodology called Dynamic Quality Function Deployment (DQFD) is put forward in this study. Then, combining with the software structure design method of Quantification Method of Type 3, DQFD is applied to control requirements change, Syncretizing the theory and method of konwledge management and an approach called software requirements change management based on DQFD is proposed. The applicability and validity of the proposed approach are testified through its application to an actual software development project, and these results further consummate the theory framework of this paper.

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