Being a key ecological security barrier and production base for grassland animal husbandry in China, the balance between grassland forage supply and livestock-carrying pressure in North China directly affects grassland degradation and restoration, thereby impacting grassland ecosystem services. This paper analyzes the spatiotemporal variation in grassland vegetation coverage, forage supply, and the balance between grassland forage supply and livestock-carrying pressure from 2000 to 2015 in North China. We then discuss the spatial pattern of grassland ecological conservation under the impacts of grassland degradation and restoration, and livestock-carrying pressure. Over the last 16 years, the total grassland area in North China decreased by about 16,000 km 2 , with vegetation coverage degraded by 6.7% of the grasslands but significantly restored by another 5.4% of grasslands. The provisioning of forage by natural grassland mainly increased over time, with an annual growth rate of approximately 0.3 kg/ha, but livestock-carrying pressure also increased continuously. The livestock-carrying pressure index without any supplementary feeding reached as high as 3.8. Apart from the potential livestock-carrying capacity in northeastern Inner Mongolia and the central Tibetan Plateau, most regions in North China are currently overloaded. Considering the actual supplementary feeding during the cold season, the livestock-carrying pressure index is about 3.1, with the livestock-carrying pressure mitigated in central and eastern Inner Mongolia. Assuming full supplementary feeding in the cold season, livestock-carrying pressure index will fall to 1.9, with the livestock-carrying pressure alleviated significantly in Inner Mongolia and on the Tibetan Plateau. Finally, we propose different conservation and development strategies to balance grassland ecological conservation and animal husbandry production in different regions of protected areas, pastoral areas, farming-pastoral ecotone, and farming areas, according to the grassland ecological protection patterns.

Thalassemia is a common monogenic disease in southwestern China, especially in Guizhou province. In this study, 18 309 neonates were examined for thalassemia. The thalassemia carrier rate was 12.90%, which is associated with geographical regions, with carrier frequencies significantly differing between regions (p < 0.0001). The carrier rates for α‐thalassemia and β‐thalassemia were 8.91% and 3.36%, respectively. There are 22 genotypes identified among 1632 α‐thalassemia cases, and 18 genotypes detected among 615 β‐thalassemia cases. The birthrates of individuals with intermediate thalassemia and β‐thalassemia major were 0.153% and 0.055%, respectively. Methodologically, NGS‐Gap‐PCR is superior to traditional detection methods, with 65 more cases detected by NGS‐Gap‐PCR. Since thalassemia‐rich genotypes were highly prevalent in this region, early detection of thalassemia carriers would be meaningful for genetic counseling and prevention/treatment of thalassemia. NGS‐Gap‐PCR provides a powerful tool for neonate genetic testing and clinical diagnosis of thalassemia, especially in high‐prevalence regions.

Until 2015, China had established 2740 nature reserves with a total area of 1.47 million km 2 , covering 14.8% of China's terrestrial land surface. Based on remote sensing inversion, ecological model simulation and spatial analysis methods, we analyzed the spatial and temporal variations of fractional vegetation coverage (FVC), net primary production (NPP), and human disturbance (HD) in habitats of typical national nature reserves (NNRs) during the first 15 years of the 21st century from 2000 to 2015. And then the three indicators were compared between different NNR types and varied climate zones. The results showed that (1) the average 5-year FVC of NNRs increased from 36.3% to 37.1%, and it improved in all types of NNRs to some extent. The annual average FVC increased by 0.11%, 0.84%, 0.21%, 0.09%, 0.11% and 0.08% in NNRs of forest ecosystem, plain meadow, inland wetland, desert ecosystem, wild animal and wild plant, respectively. (2) The NPP annually increased by 2.06 g•m-2 , 1.23 g•m-2 , 0.28 g•m-2 and 0.4 g•m-2 in NNRs of plain meadow, inland wetland, desert ecosystem and wild animal, respectively. However, it decreased by 3.45 g•m-2 and 2.35 g•m-2 in NNRs of forest ecosystem and wild plant respectively. (3) In the past 15 years, besides the slight decreases in the NNRs located at the Qinghai-Tibet Plateau and the south subtropical zone, HD enhanced in most of NNRs, especially HD in the warm temperate humid zone increased from 4.7% to 5.35%.

Protected areas (PAs) provide refuges for threatened species and are considered to be the most important approach to biodiversity conservation. Besides climate change, increasing human population is the biggest threat to biodiversity and habitats in PAs. In this paper, the temporal and spatial variations of land cover changes (LCC), vegetation fraction (VFC), and net primary productivity (NPP) were studied to present the ecosystem dynamics of habitats in 6 different types of national nature reserves (NNRs) in 8 climate zones in China. Furthermore, we used Defense Meteorological Satellite Program/Operational Linescan System (DMSP/OLS) nighttime light datasets and the human disturbance (HD) index estimated from LCC to quantify the living and developing human pressures within the NNRs in the period 2000–2013. The results showed that (1) the living human activities of NNRs increased apparently in the humid warm-temperate zone, Qinghai-Tibet Plateau, mid-temperate semi-arid zone, and mid-temperate humid zone, with the highest increase of nighttime light observed in inland wetlands; (2) the developing human activities in NNRs indicated by the HD index were higher in the humid warm-temperate zone and mid-temperate semi-arid zone as a result of increasing areas of agricultural and built activities, and lower in the sub-tropics due to improved conservation of forest ecosystems; (3) the relationship between HD and VFC suggests that ecosystems in most NNRs of south-subtropics, mid-temperate arid zone and Qinghai-Tibet Plateau were predominantly impacted by climate change. However, HDs were the prevalent factor of ecosystem dynamics in most NNRs of north-subtropics, mid-temperate semi-arid and humid zones.