Helicobacter pylori (H. pylori) is the main pathogenic factor of gastric cancer, chronic gastritis, and other gastric diseases. It has been found that Callicarpa nudiflora (CN) as an air-dried leaf extract has a broad-spectrum antibacterial effect. This study aims to examine the effect of CN on H. pylori-infected GES-1 cells in vitro and elucidate its underlying mechanism by extracting active ingredients from air-dried leaves. GES-1 cells were cocultured with HPSS1 at MOI = 100 : 1 and treated with different concentrations of CN (100 and 200 μg/ml). Results showed that CN can significantly reduce cellular LDH leakage and attenuate H. pylori-induced cell apoptosis and ROS production in GSE-1 cells, so as to protect gastric epithelial cells from damage by H. pylori. CN can also inhibit the secretion of inflammatory factors, such as TNF-α, IL-1β, IL-6, and IL-8. After CN treatment, the expression levels of active caspase-1, PYCARD, and NLRP3 were remarkably decreased in the treatment groups compared with the model group. To sum up, CN is highly protective against H. pylori-induced cell damage and apoptosis; CN can depress NLRP3 inflammasome activation and ROS production via the ROS/NLRP3/caspase-1/IL-1β signaling axis to suppress H. pylori-triggered inflammatory response and pyroptosis.

Background and objectives:Studies that have investigated the effects of curcumin on the levels of IL-6 and IL-8 are controversial. We performed this meta-analysis to quantitatively evaluate the effects of curcumin on IL-6 and IL-8 regulation.Methods and study design: The PubMed, Embase, and Cochrane Library (updated to March 2017) databases were searched for related studies. The pooled effects of curcumin treatment on IL-6 and IL-8 were evaluated using a random-effects model. Nine studies comprising a total of 512 subjects were included in the current meta-analysis. Results:The overall outcome of this meta-analysis suggested that curcumin had no favorable effects on the concentration of IL-6 (-0.80 pg/mL; 95% CI, -1.61 to 0.01 pg/mL; P=0.052). Compared with control subjects, subjects treated with curcumin did not show a significant decrease in the concentration of IL-8 (-0.20 pg/mL; 95% CI, -1.02 to 0.62 pg/mL; P=0.639). Subgroup analysis further confirmed that the overall effects of curcumin on the concentrations of IL-6 and IL-8 were not notably affected by the dose or intervention duration of curcumin. Meta-regression analyses did not indicate dose effects of curcumin on IL-6 and IL-8. Conclusion:This meta-analysis suggested that treatment of curcumin did not significantly reduce the concentrations of IL-6 and IL-8.

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Helicobacter pylori ( H. pylori ) is a major pathogenic factor for the development of gastric diseases including chronic gastritis and gastric cancer. Callicarpa nudiflora (CN), an air-dried leaves extract of Callicarpa nudiflora Hook. & Arn., has been found to exhibit a broad-spectrum antibacterial effect. In our study, we extracted the active ingredient from air-dried leaves of Callicarpa nudiflora, detected the effect of CN against H. pylori -infected GES-1 cells in vitro , and elucidated the underlying mechanism. GES-1 cells were cocultured with HPSS1 at MOI = 100:1 and treated with different concentrations of CN. Results indicated that CN not only significantly decreased cellular lactate dehydrogenase leakage, but also markedly attenuated H. pylori -induced cell apoptosis and ROS production in GSE-1 cells, therefore protecting gastric epithelial cells against injuries caused by H. pylori . CN also inhibited the secretions of inflammatory factors, such as tumor necrosis factor-α (TNF-α), IL-1β, IL-6 and IL-8. Furthermore, CN remarkably decreased the expression levels of NLRP3, PYCARD, active Caspase-1. In conclusion, CN exhibited highly efficient protective effect against H. pylori -induced gastritis and cell damage; Mechanismly, CN suppressed H. pylori -triggered inflammatory response and pyroptosis through depressing ROS production and NLRP3 inflammasome activation via ROS/NLRP3/IL-1β signaling axis.

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