Few studies have been reported that focus on developing implant surface nanofiber (NF) coating to prevent infection and enhance osseointegration by local drug release. In this study, coaxial doxycycline (Doxy)-doped polycaprolactone/polyvinyl alcohol (PCL/PVA) NFs were directly deposited on a titanium (Ti) implant surface during electrospinning. The interaction of loaded Doxy with both PVA and PCL NFs was characterized by Raman spectroscopy. The bonding strength of Doxy-doped NF coating on Ti implants was confirmed by a stand single-pass scratch test. The improved implant osseointegration by PCL/PVA NF coatings in vivo was confirmed by scanning electron microscopy, histomorphometry and micro computed tomography (μCT) at 2, 4 and 8 weeks after implantation. The bone contact surface (%) changes of the NF coating group (80%) is significantly higher than that of the no NF group (<5%, p < 0.05). Finally, we demonstrated that a Doxy-doped NF coating effectively inhibited bacterial infection and enhanced osseointegration in an infected (Staphylococcus aureus) tibia implantation rat model. Doxy released from NF coating inhibited bacterial growth up to 8 weeks in vivo. The maximal push-in force of the Doxy-NF coating (38 N) is much higher than that of the NF coating group (6.5 N) 8 weeks after implantation (p < 0.05), which was further confirmed by quantitative histological analysis and μCT. These findings indicate that coaxial PCL/PVA NF coating doped with Doxy and/or other drugs have great potential in enhancing implant osseointegration and preventing infection.

Coffea arabica L. is a famous specie in genus caffee for medicinal treatment and diet with wide distribution and rich resources. It contains rich alkaloids, flavonoids and terpenes, which exhibited antioxidation, anti-inflammatory, antitumor, antidiabetic, live protection, and neuroprotective activities. Herein, we summarized the progress in the chemical constituents and bioactivities of C. arabica L. to provide ideas for medicinal development prospects of C. arabica L.

Aconitum episcopale Levl., known as a characteristic Aconitum specie in China, is a perennial herbaceous plant classified into Aconitum L. (Ranunculaceae), which mainly distributes in northwest Yunnan and southwestern Sichuan. As a commonly traditional Chinese medicine, the root of A. episcopale has been used to treat anemofrigid-damp arthralgia, arthralgia, cold hernia and anesthesia analgesia with the effects of expelling wind and removing damness, warming menstruation and relieving pain. It also is used for solving other Aconitums poisoning in folk. In order to obtain more information for research and development of A. episcopale, the chemical components and pharmacological research on A. episcopale were reviewed. It is beneficial for future research, development and utilization of Aconitum and search for traditional herbal resources.