Hydrolysis of racemic acetates with Pseudomonas fluorescens lipase (PFL) afforded optically active alcohols with the R-configuration, independent of ring size; a three-site model for PFL-catalysed hydrolysis is proposed.
The racemic acetates of five-membered-ring alcohols were successfully resolved into the optically active alcohols with high optical purities by Pseudornonas fluorescens lipase.
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