Bamboos seldom bloom and almost no seeds could be harvested, and, hence, few works are focused on germination physiology. Systematic research on the physiological effects of colchicine on germination and seedling growth of bamboo seeds is lacking. In this study, we finely recorded seed germination and seedling growth of Dendrocalamus brandisii in media supplemented with different colchicine concentrations. Physiological effects and mechanisms of colchicine were analyzed. The results showed that D. brandisii seeds were non-dormant, and seed lots achieved their highest germination rates on the 4th day and finished the whole germination period after 21 days. Colchicine inhibited seed germination and seedling growth but did not change its germination pattern. Seed germination and seedling growth decreased constantly with colchicine concentration. Colchicine showed more negative effects on seedling growth than on seed germination and root growth. High concentrations of colchicine retarded the development of plumules and even caused their aberrant development. Under tissue culture conditions, seed germination, and seedling growth relied mainly on the endogenous starch and soluble sugar degradation, in which α-amylase, STP, and SUSY played the key role. Colchicine inhibited seed germination and seedling growth by suppressing the α-amylase, STP, and SUSY activities. Colchicine showed more negative effects on sucrose degradation than on starch degradation during seed germination and seedling growth. This study provides new basic information on the seedling physiology for the genetic breeding of bamboo plants.

Only a few calli regeneration systems of bamboos were successfully established, which limited the research on physiological mechanism of callus differentiation. In this study, we successfully established the callus differentiation systems of Dendrocalamus brandisii via seeds. The results showed that the best medium for callus induction of D. brandisii seeds was basal MS media amended with 5.0 mg L−1 2,4-D and 0.5 mg L−1 KT, and the optimal medium for shoot differentiation was the basal MS media supplemented with 4.0 mg L−1 BA and 0.5 mg L−1 NAA. Callus tissues had apparent polarity in cell arrangement, and developed their own meristematic cell layers. α-amylase, STP and SUSY played a dominant role in carbohydrates degradation in callus during shoot differentiation. PPP and TCA pathways up-regulated in the shoot-differentiated calli. The dynamics of BA and KT contents in calli was consistent with their concentrations applied in medium. IAA synthesis and the related signal transduction were down-regulated, while the endogenous CTKs contents were up-regulated by the exogenous CTKs application in shoot-differentiated calli, and their related synthesis, transport and signal transduction pathways were also up-regulated. The downregulated signal transduction pathways of IAA and ABA revealed that they did not play the key role in shoot differentiation of bamboos. GAs also played a role in shoot differentiation based on the down-regulation of DELLA and the up-regulation of PIF4 genes. The overexpression of DbSNRK2 and DbFIF4 genes further confirmed the negative role of ABA and the positive role of GAs in shoot differentiation.