In recent years, gold nanoparticles (GNPs) have gradually become a major choice of drug delivery cargoes due to unique properties. Compared to traditional bulk solid gold, GNPs have basic physical and chemical advantages, such as a larger surface area-to-volume ratio and easier surface modification. Furthermore, these have excellent biocompatibility, can induce the directional adsorption and enrichment of biological macromolecules, help retain biological macromolecule activity, and cause low harm to the human body. All these make GNPs good drug delivery cargoes. The present study introduces the properties of GNPs, including factors that affect the properties and synthesis. Then, focus was given on the application in drug delivery, not only on the molecular mechanism, but also on the clinical application. Furthermore, the properties and applications of peptide GNPs were also introduced. Finally, the challenges and prospects of GNPs for drug delivery were summarized.

Dental-derived stem cells have excellent proliferation ability and multi-directional differentiation potential, making them an important research target in tissue engineering. An increasing number of dental-derived stem cells have been discovered recently, including dental pulp stem cells (DPSCs), stem cells from exfoliated deciduous teeth (SHEDs), stem cells from apical papilla (SCAPs), dental follicle precursor cells (DFPCs), and periodontal ligament stem cells (PDLSCs). These stem cells have significant application prospects in tissue regeneration because they are found in an abundance of sources, and they have good biocompatibility and are highly effective. The biological functions of dental-derived stem cells are regulated in many ways. Epigenetic regulation means changing the expression level and function of a gene without changing its sequence. Epigenetic regulation is involved in many biological processes, such as embryonic development, bone homeostasis, and the fate of stem cells. Existing studies have shown that dental-derived stem cells are also regulated by epigenetic modifications. Pulp and periodontal regeneration refers to the practice of replacing damaged pulp and periodontal tissue and restoring the tissue structure and function under normal physiological conditions. This treatment has better therapeutic effects than traditional treatments. This article reviews the recent research on the mechanism of epigenetic regulation of dental-derived stem cells, and the core issues surrounding the practical application and future use of pulp and periodontal regeneration.

Oral ulcers are one of the most common inflammatory diseases on oral mucosa that have obvious impacts on patients. Studies have shown that N6-methyladenosine (m6A) RNA transcription modification may be involved in the development of various inflammatory responses, and whether the pathogenesis of oral ulcers is related to m6A is unclear. This study aims to identify how m6A-related single nucleotide polymorphisms (m6A-SNPs) may affect oral ulcers. The UKBB dataset containing 10,599,054 SNPs was obtained from the GWAS database using the keyword “oral ulcer” and compared with the M6AVar database containing 13,703 m6A-SNPs.With 7,490 m6A-SNPs associated with oral ulcers identified, HaploReg and RegulomeDB were used for further functional validation and differential gene analysis was performed using the GEO database dataset GSE37265. A total of 7490 m6A-SNPs were detected in this study, 11 of which were related to oral ulcers (p<5E-08), and all of these SNPs showed eQTL signals. The SNP rs11266744 (p=2.00E-27) may regulate the expression of the local gene CCRL2, thereby participating in the pathogenesis of oral ulcers. In summary, by analyzing genome-wide association studies, this study showed that m6A modification may be involved in the pathogenesis of oral ulcers and CCRL2 may be the targeted gene.

Dental-derived stem cells, an important research target in tissue engineering, have excellent proliferation ability and multi-directional differentiation potential. In recent years, an increasing number of odontogenic stem cells have been discovered, including dental pulp stem cells (DPSCs), stem cells from exfoliated deciduous tooth (SHEDs), apical papilla stem cells (SCAPs), dental follicle precursor cells (DFPCs) and periodontal ligament stem cells (PDLSCs). Due to the advantages of abundant sources, safe and effective, these stem cells have significant application prospects in tissue regeneration. The biological functions of odontogenic stem cells are regulated in many ways. Epigenetic regulation means changing the expression level and function of a gene without changing its sequence. Epigenetic regulation is involved in many biological processes, such as embryonic development, bone homeostasis, and stem cell fate. Existing studies have shown that odontogenic stem cells are also regulated by epigenetic modifications. Referring to replacing damaged pulp and periodontal tissue and restoring the tissue structure and function under normal physiological conditions, pulp regeneration and periodontal regeneration has better therapeutic effect than traditional treatments. This article reviews the recent research progress on the epigenetic regulation mechanism of odontogenic stem cells, and describes the potential applications of odontogenic stem cells-based epigenetic regulation in dental pulp and periodontal regeneration.