An enantioselective Pd(II)-catalyzed amino-cyclization and desymmetrizing nitrile addition cascade reaction of alkyne-tethered malononitriles is reported. This reaction forms two rings and one quaternary carbon center in a single step and serves as an efficient strategy for the construction of α-quaternary carbazolones with high enantioselectivities (up to 98:2 er). The utility of this method is demonstrated by product derivatization into a diverse array of heterocycles and a nitrile-containing leucomidine A analog.
Efficacy evaluation through human trials is crucial for advancing a vaccine candidate to clinics. Next-generation sequencing (NGS) can be used to quantify B cell repertoire response and trace antibody lineages during vaccination. Here, we demonstrate this application with a case study of Hecolin®, the licensed vaccine for hepatitis E virus (HEV). Four subjects are administered the vaccine following a standard three-dose schedule. Vaccine-induced antibodies exhibit a high degree of clonal diversity, recognize five conformational antigenic sites of the genotype 1 HEV p239 antigen, and cross-react with other genotypes. Unbiased repertoire sequencing is performed for seven time points over six months of vaccination, with maturation pathways characterize for a set of vaccine-induced antibodies. In addition to dynamic repertoire profiles, NGS analysis reveals differential patterns of HEV-specific antibody lineages and highlights the necessity of the long vaccine boost. Together, our study presents a quantitative strategy for vaccine evaluation in small-scale human studies.
An enantioselective nickel-catalyzed
intramolecular reductive cross-coupling
of C(sp2) electrophiles and cyano groups is reported. Enantioenriched
CN-containing all-carbon quaternary stereocenters are assembled by
desymmetrizing cyclization of aryl/alkenyl halide-tethered malononitriles.
The use of an organic reductant, (EtO)2MeSiH, is crucial
to the enantioselectivity and reactivity. Applications of the method
are demonstrated through the synthesis of bioactive molecules and
their cyanated analogues and the total synthesis of the natural product
diomuscinone. This study exhibits the potential of desymmetrizing
reductive coupling strategies to access structurally rigid and synthetically
versatile molecules from readily available starting materials.
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