Chlorella pyrenoidosa has been used safely in humans and in other animals as an immune stimulant. The aim of this study was to evaluate the dietary supplementation with Chlorella pyrenoidosa to ameliorate the effects of subacute intoxication with aflatoxin B1 (AFB1) in broiler chickens. Hepato-protective assessment of 7-d-old chicks was performed on a total of 120 chickens (Hubbard) divided into six groups having two replicates with 10 birds each: Control, AFB1 (350 ppb), Chlorella pyrenoidosa ethanolic extract (CP) at 250 mg; 500 mg and cotreatment of CP (250 mg; 500 mg) and AFB1. The experiment was conducted for 35 days. The contents of AFB1 and AFB1+CP diets were 350 ppb throughout the experimental period. The results indicated that diet contaminated with low level of AFB1 significantly (P<0.05) reduced the average weight gain and daily food intake during the entire experiment and decreased (P<0.05) the serum contents of total protein, albumin and globulins. Moreover, a dietary low level of AFB1 not only increased (P<0.05) lipid peroxidation but also reduced (P<0.05) total antioxidant capability, catalase, glutathione reductase and peroxidase activity in liver. Furthermore, addition of CP (500 mg) to AFB1 contaminated diet counteracted these deleterious effects, indicating that supplementation of Chlorella pyrenoidosa to the broiler diet was safe, quiet cheaper provides 16% cost benefit ratio as compared to standard feed supplement, promoting beneficial effects (500 mg) in poultry health with respect to the toxic effects of 350 ppb diet.

The present study was designed to investigate effect of Oxalis corniculata (Creeping wood sorrel) against aflatoxicosis in straight run broiler chicken (Hubbard strain). Hundred and twenty-day old broiler chicks were randomly divided into six groups with two replicates. Two different levels of O.corniculata (250; 500 mg/kg b.w) with and without AFB1 (350 ppb) and a control treatment were added to the diet. Dietary treatment initiated at the end of 1 st week and sustained for six weeks. O.corniculata supplementation in the diet during five weeks significantly reduced body weight, cumulative feed intake and feed conversion ratio of the treated groups in dose dependent manner. Dietary incorporation of O.corniculata 250 mg to 500mg induced negative effects on overall broiler health performance. KEYWORDSOxalis corniculate, aflatoxicosis, cumulative feed intake, feed conversion ratio.