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The core-shell structure nanofibers of poly(ethylene oxide)/chitosan have been electrospun from the homogeneous solution of chitosan (CS, as shell) and poly(ethylene oxide) (PEO, as core). The preparation process of core-shell structure was quite simple and efficient without any complex electrospinning setup or post-treatment. The core-shell structure and major component of each layer had been characterized by TEM and further supported by SEM, XRD, DSC, and EDS studies. The blending ratio of PEO and CS, molecular weight of chitosan, and temperature of electrospinning were thought to be the key influence factors on the formation of core-shell structure. Because of the chitosan outer layer and shell thickness being controllable, the core-shell structure nanofiber would show a potential application for the biomedical fields involving wound care and tissue engineering.

The red‐spotted grouper Epinephelus akaara (E. akaara) is one of the most economically important marine fish in China, Japan and South‐East Asia and is a threatened species. The species is also considered a good model for studies of sex inversion, development, genetic diversity and immunity. Despite its importance, molecular resources for E. akaara remain limited and no reference genome has been published to date. In this study, we constructed a chromosome‐level reference genome of E. akaara by taking advantage of long‐read single‐molecule sequencing and de novo assembly by Oxford Nanopore Technology (ONT) and Hi‐C. A red‐spotted grouper genome of 1.135 Gb was assembled from a total of 106.29 Gb polished Nanopore sequence (GridION, ONT), equivalent to 96‐fold genome coverage. The assembled genome represents 96.8% completeness (BUSCO) with a contig N50 length of 5.25 Mb and a longest contig of 25.75 Mb. The contigs were clustered and ordered onto 24 pseudochromosomes covering approximately 95.55% of the genome assembly with Hi‐C data, with a scaffold N50 length of 46.03 Mb. The genome contained 43.02% repeat sequences and 5,480 noncoding RNAs. Furthermore, combined with several RNA‐seq data sets, 23,808 (99.5%) genes were functionally annotated from a total of 23,923 predicted protein‐coding sequences. The high‐quality chromosome‐level reference genome of E. akaara was assembled for the first time and will be a valuable resource for molecular breeding and functional genomics studies of red‐spotted grouper in the future.