PURPOSE: Color blindness is one of the potential disabilities affecting the ability of color perception by the eye. The aim of the present study was to evaluate the prevalence of color blindness among dental professionals. METHODS: For the present study, a total of 198 dental professionals were randomly selected as subjects who were asked to fill the required questionnaire followed by which their color vision status was evaluated using the Ishihara test. The results obtained were subjected to statistical analysis. Statistical analysis was performed using the Statistical Package for the Social Sciences (SPSS) version 17.0 (SPSS Inc., Chicago, IL, USA). The prevalence of color blindness, age specificity, and the ratio of the occurrence of color blindness in relation to gender were assessed using Chi-square test for independence and Chi-square test with Yates's correction for independence when the expected frequency was <5. P < 0.05 was considered statistically significant. RESULTS: The present study reported 3.54% prevalence of color blindness among the study population. A higher number of cases were reported from males (9.26%) than females (1.39%). ( P < 0.05) Furthermore, the prevalence of color blindness was found to be more in faculty members (5.88%) than in students with a reported prevalence of 3.31%. CONCLUSION: The present study reported high prevalence of color blindness in dental professionals while they were totally unaware of it.