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Abstract-In favour of the reconstruction of the real or virtual image's phase and intensity, the complete digital holographic images are being processed. This reconstruction takes place with the numerical definition of the diffraction integral. One of the possible realization is the Fresnel approximation, which employs a sole Fourier-transformation. Another method is to interpret the diffraction formula as a convolution integral, and if we calculate the formula, it will be doubled or tripled because of the transformation. The impulse response of wave fields should be represented in this convolution approach, from which the Fourier transform can be immediately determined. The impulse response as well as the Fourier transform can be immediately specified, or well approximated. The essential distinction between the Fresnel and convolution approach is the different size of resultant images. Furthermore, this size in case of the Fresnel process depends on the distance of the object and the sensor, as well as the wavelength of the illuminating light; but in the other case, it is invalid.

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With holographic microscopy, high-resolution digital images can be taken of smaller objects. The technology exploits the wave feature of light in a way that even a threedimension image can be taken, therefore, the examined objects spatial structure can also be defined. The holographic application can eliminate one of the biggest deficiency of microscopes, the depth of focus [1]. Therefore, it can be applied at processes, in which the examined objects shape, extension may carry additional information. Such processes can be, for instance, the examination of liquids, or blood. Through the process of liquid-examination, the shaped elements spatial extension can be allocated as well.

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