Endophytic phosphorus-and potassium-solubilizing bacteria were screened from the root, rhizome, stem, and leaves of Moso Bamboo, and their diversity was analyzed using their 16S rDNA sequences. Twenty endophytic phosphorus and potassium-solubilizing bacteria were screened from 82 bamboo plants, among which the CT-B09-2, WYS-A01-1 and JL-B06 had higher activities in decomposing organophosphates. The three species showed a decomposition diameter/colony diameter (D/d) of 5.05, 4.19 and 2.95, respectively, and a solubilizing activity of 81.77 mg/L, 77.85 mg/L and 63.69 mg/L, respectively. JL-B06, WYS-A01-1 and CT-B09-2 had higher activities in decomposing inorganic phosphorus, with a decomposition diameter/colony diameter (D/d) of 2.34, 2.12 and 1.82, respectively, and a solubilizing activity of 30.58 mg/L, 38.89 mg/L and 48.35 mg/L, respectively. CT-B21, WYS-A03-1 and JL-B06 had higher activities in decomposing potassium, with a decomposition diameter/colony diameter (D/d) of 3.37, 4.84 and 4.33, respectively, and a solubilizing activity of 2.81 mg/L, 2.54 mg/L and 2.46 mg/L, respectively. The 16S rDNA sequence analysis showed that the 20 phosphorus-and potassium-solubilizing bacteria belong to 14 species from 10 genera, and mainly consist of Alcaligenes spp., Enterobacter spp. and Bacillus spp. Our results demonstrate the abundant diversity of endophytic phosphorus-and potassiumsolubilizing bacteria in Moso Bamboo.

The plant microbiota play a key role in plant productivity, nutrient uptake, resistance to stress and flowering. The flowering of moso bamboo has been a focus of study. The mechanism of flowering is related to nutrient uptake, temperature, hormone balance and regulation of key genes. However, the connection between microbiota of moso bamboo and its flowering is unknown. In this study, samples of rhizosphere soil, rhizomes, roots and leaves of flowering and nonflowering plants were collected, and 16S rRNA amplicon Illumina sequencing was utilized to separate the bacterial communities associated with different flowering stages of moso bamboo. We identified 5442 OTUs, and the number of rhizosphere soil OTUs was much higher than those of other samples. Principal component analysis (PCA) and hierarchical clustering (Bray Curtis dis) analysis revealed that the bacterial microorganisms related to rhizosphere soil and endophytic tissues of moso bamboo differed significantly from those in bulk soil and rhizobacterial and endosphere microbiomes. In addition, the PCA analyses of root and rhizosphere soil revealed different structures of microbial communities between bamboo that is flowering and not flowering. Through the analysis of core microorganisms, it was found that Flavobacterium, Bacillus and Stenotrophomonas played an important role in the absorption of N elements, which may affect the flowering time of moso bamboo. Our results delineate the complex host-microbe interactions of this plant. We also discuss the potential influence of bacterial microbiome in flowering, which can provide a basis for the development and utilization of moso bamboo.

We analyzed culturable endophytic bacteria from Moso bamboo (Phyllostachys edulis) using traditional bacterial isolation and culture methods and then studied the colony characteristics and diversity with a 16S rDNA sequence analysis. We isolated 82 endophytic bacteria strains belonging to 47 species in 26 genera from the root, rhizome, stem and leaves of Moso bamboo species from populations on Wuyi Mountain, and in the Jiangle and Changting regions. There were significant differences in the composition of the culturable endophytic bacteria isolated from the different areas and from different tissues. The dominant bacteria strains from the Wuyi Mountain samples were Arthrobacter, Staphylococcus, Bacillus and Enterobacter, while the dominant bacteria from the Jiangle samples were Bacillus, Staphylococcus and Curtobacterium, and the dominant bacteria in the Changting samples were Alcaligenes, Pseudomonas, Staphylococcus and Bacillus. Our results demonstrate the abundant diversity of endophytic bacteria in Moso bamboo.

The research results of the growth-promoting effects of endophytic bacteria on Phyllostachys edulis indicated that the growth-promoting endophytic bacteria could improve photosynthesis in P. edulis leaves. The photosynthetic rate, transpiration rate, and the stomatal conductance in P. edulis treated with endophytic bacteria were all higher than in the control group. Endophytic bacteria could also increase the chlorophyll content and the protective enzyme activities in P. edulis, improving their reactions to the adverse environmental conditions. Through injection treatments with growth-promoting endophytic bacteria, the catalase, superoxide dismutase (SOD), peroxidase activity, soluble protein content, and soluble sugar content in P. edulis were all higher than in the control group, except for the malondialdehyde content, which was lower than in the control group.

To understand the diversity and distribution of endophytic bacteria in moso bamboo (Phyllostachys edulis), we used 16S rDNA sequencing to investigate the characteristics and diversity of endophytic bacteria in different moso bamboo tissues. After 454 pyrosequencing, we obtained 141,269 sequences from seven moso bamboo tissue samples. The taxonomic origins of unique sequences were identified using RDP classifier. The results showed that these sequences belonged to 26 bacterial orders, including the Actinomycetales, Rickettsiales, Burkholderiales, Enterobacteriales, and Rhizobiales. Among these, Enterobacteriales was widely found in all bamboo tissues. Endophytic bacterial communities differed between the moso bamboo shoot and pole. With continuous growth and development, the number of endophytic species in the moso bamboo pole increased gradually.