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Tumor necrosis factor alpha-induced protein 8-like 2 (TNFAIP8L2) is a new member of the tumor necrosis factor-alpha-induced protein 8 (TNFAIP8) family that functions as an important factor in the maintenance of immune homeostasis. In this study, we cloned the cDNA sequences and analyzed the genomic structure of porcine TNFAIP8L2. RH mapping using the IMpRH panel showed that this gene was closely linked to microsatellite marker SW512 in pig chromosome 4. Subcellular localization analysis showed GFP-TNFAIP8L2 fusion protein distributed in nucleus as well as cytoplasm including mitochondria and endoplasmic reticulum. Real-time PCR analysis revealed that porcine TNFAIP8L2 was more highly expressed in spleen than other tissues. To understand its characterization of transcriptional regulation, we cloned approximately 2 kb of 5'-regulatory region upstream to the porcine TNFAIP8L2 translational start site and generated sequential deletion constructs evaluated in dual-luciferase reporter assay. The results demonstrated that its core promoter is 435 base pairs (bp) upstream to the transcription initiation site. Then, site-directed mutation experiment combined with electrophoretic mobility shift assay (EMSA) indicated that M-CAT binding factor (MCBF) and activator protein 1 (AP-1) were important transcription factors for porcine TNFAIP8L2. These findings provide an important basis for further understanding of porcine TNFAIP8L2 regulation and function in swine.

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Inherent brittleness is the most weakness of bismaleimide resin, which adversely affects the performance and application of the terminal fiber reinforced bismaleimide composites. In this study, polyhedral oligomeric silsesquioxane (POSS) with glycidyl group (G-POSS) and methacryloxy propyl group (MA-POSS) are utilized to toughen diallyl bisphenol-A/N, N 0 -4,4 0 -diphenylmethane bismaleimide (BD resin) to obtain hybrid resins named BDGP and BDMP. The results of hybrid resins show that the two series hybrid resins perform higher toughness, strength, water resistance as well as lower dielectric constant and loss comparing with pristine BD resin, and BDMP perform better than BDGP due to that MA-POSS can react with BD resin while G-POSS cannot. In specific, the BDMP 3 in which 3 wt% MA-POSS is added performs the highest impact strength of 19.0 KJ/m 2 , increased by 102.1% comparing with pristine BD resin, and it also performs the lowest dielectric constant of 3.04 as well as lowest dielectric loss of 0.006 at 1 MHz. In addition, the silica fiber reinforced BDMP composites (SF/BDMP) are fabricated, and the results show that the bending strength and interlaminar shear strength of SF/BDMP 3 composite are enhanced to 290.2 and 32.5 MPa, 32.0% and 38.4% higher than those of SF/BD composite, and the corresponding dielectric loss (0.025) at 8.2 GHz is reduced by 45.9%. This research displays that reactive POSS can improve the toughness and reduce the dielectric loss of resin, which is benefit to obtain a high performance and functional resin-based composites with excellent mechanical properties and low dielectric properties.bismaleimide, low dielectric loss, polyhedral oligomeric silsesquioxane, SF/bismaleimide composites, toughness | INTRODUCTIONBismaleimide resin (BMI) is a commercial thermosetting resin with excellent strength, water resistance, thermal stability, and electrical insulation properties, [1][2][3] and it can be reinforced by silica fiber (SF) to fabricate wavetransparent composites with low dielectric constant and loss to meet the application in aviation, aerospace, highperformance electronic devices, printed circuit boards and other key areas. [4][5][6] However, due to the high cross-