Objectives: To evaluate the diagnostic value and clinical application of prenatal ultrasonography (US) and Magnetic Resonance Imaging (MRI) for different types of fetal Agenesis of the Corpus Callosum (ACC). Material and methods: There were 42 cases of fetal ACC discovered by routine US, including complete ACC 18 cases and partial ACC 24 cases, checked by MRI within 1 week. The results were confirmed by head ultrasound after birth or brain biopsy after labor induction. Results: From prenatal ultrasonic diagnosis, 18 cases were complete ACC and 24 cases were partial ACC. MRI was able to find complete ACC in 11 cases, partial ACC in 16 cases, and non-ACC in 15 cases. Labor induction or birth confirmed that, 11 cases were complete ACC, 14 cases were partial ACC, and 17 cases were non-ACC.The results of different types of ACC were detected by ultrasound and MRI were statistically significant (p < 0.05).MRI examination was superior to ultrasound in specificity, positive predictive value, negative predictive value, Youden index, and diagnostic index. Conclusions: MRI is high specific degrees, diagnostic performance is satisfactory, should be use as a necessary method for prenatal definitive diagnosis of ACC. However, prenatal ultrasound can be tested repeatedly and can be combined with blood flow imaging detection in real time, and it is still the preferred method for screening fetal structural malformation in a comprehensive way, which is suitable for general screening of ACC.