Antibiotic resistance is now a global health issue. Antibiotic abuse leads microorganisms to grow resistant to a variety of antibiotics. Antibiotic resistance and resistant genes in Klebsiella pneumoniae in broiler chickens in West Java Province were investigated. Broilers from Bogor and Sukabumi were used to obtain 200 cloacal swab samples. Cultures on MacConkey agar, Gram staining, biochemistry test, and polymerase chain reaction (PCR) were used to isolate and identify the samples. The Kirby-Bauer disk diffusion method was used to investigate antibiotic sensitivity, and PCR was used to detect resistance genes. A total of 20% of the samples tested positive for K. pneumoniae. Among other drugs, K. pneumoniae isolates demonstrated resistance to erythromycin (100%), oxytetracycline (97.5%), ampicillin (97.5%), tetracyclines (95%), nalidixic acid (95%), enrofloxacin (EN) (87.5%), EN (82.5%), ciprofloxacin (75.0%), gentamicin (45.0%), and chloramphenicol (25%). All isolates (100.0%) had gyrA and blaTEM genes, 85.0% had tetA genes, and 52.5% had ermB genes, according to the molecular test. Klebsiella pneumoniae may be isolated and identified from broiler chickens in West Java, Indonesia, according to this study. Klebsiella pneumoniae isolated possessed gyrA, blaTEM, tetA, and ermB-resistant genes and was multidrug-resistant.

West Java province has largest population of chicken poultry, with Bogor, Sukabumi, and Cianjur has highest chicken population. Farmers used antibiotics for prophylaxis and therapy to maintain the production. However, extensive use of antibiotic increased the number of antibiotic resistant bacteria. Staphylococcus aureus, Streptococcus spp, and Klebsiella spp are the example of flora normal in chicken that affected with abusive use of antibiotic. The aim of this study was to determine the antibiotic resistance profile of S. aureus, Streptococcus spp., and Klebsiella spp. isolated from cloacal swab of chicken poultry in 3 regions. Total of 320 samples were collected and the positive number of S. aureus, Streptococcus spp., and Klebsiella spp were 61, 8, and 58 isolates respectively. The result of antibiotics susceptibility test showed that S. aureus was resistant to ampicillin (98%), erythromycin (95%), nalidixic acid (93%), tetracycline (92%), oxytetracycline (90%), enrofloxacin (69%), and ciprofloxacin (56%). Streptococcus spp. was resistant to tetracycline (100%) and doxycycline (87.5%). Klebsiella spp. was resistant to erythromycin (100%), ampicillin (94.83%) oxytetracycline (93.10%), tetracycline and nalidixic acid (89.66%), enrofloxacin (86.21%), and ciprofloxacin (81.03%). S. aureus, Streptococcus spp., and Klebsiella spp. has a high level of resistance to antibiotics and most of the isolates were multi-resistant.