Objective:The aim of this study was to determine the prevalence of Entamoeba histolytica in patients suffering from diarrhoea in Quetta. Study Design: Microscopic examination of stool samples and analysis through the formalin ethyl-acetate sedimentation technique. Duration: Six months (from 13-2-2017 to 21-7-2017). Methodology: A total of 235 stool samples randomly collected from females, males and children having abdominal discomfort who visited outpatient departments (OPDs) in different hospitals of Quetta. Results: Out of a total of 235 patients, E. histolytica was found in 45 (19.1%) patients (males = 55.6% and females = 44.4%). Conclusion: Infections of E. histolytica can be controlled by protecting food from flies, use of boiled drinking water, proper washing of fresh vegetables, hygienic conditions and a well-established sanitation system. It has been concluded that the use of animal compost for fertilizer, cleansing of vegetables with water contaminated by animal faeces, and a poor sewage system can enhance the risks of infections caused by this parasite.