Aim: The main of this study was to investigate the role of IGF2BP2 in oral squamous cell carcinoma (OSCC) with the overarching of providing new biomarkers or potential therapeutic targets for OSCC. Methods: We combined datasets downloaded from Gene Expression Omnibus (GEO), The Cancer Genome Atlas (TCGA), and samples collected from the clinic to evaluate the expression of IGF2BP2 in OSCC. IGF2BP2 survival analysis was respectively performed based on TCGA, GEO, and clinical samples. Correlations between IGF2BP2 expression and clinicopathological parameters were then analyzed, and signaling pathways associated with IGF2BP2 expression were identified using gene set enrichment analysis (GSEA 4.1.0). Moreover, an IGF2BP2 co-expressed gene network was constructed, followed by gene ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis on IGF2BP2 co-expressed genes. Finally, TIMER and CIBERSORT were used to analyze the correlations among IGF2BP2, IGF2BP2-coexpressed genes, and tumor-infiltrating immune cells (TICs). Results: IGF2BP2 was highly expressed in OSCC and significantly correlated with overall survival of OSCC patients (p＜0.01). High IGF2BP2 expression correlated with poor overall survival. The GSEA results showed that cell apoptosis-, tumor-, and immune-related pathways were significantly enriched in samples with high IGF2BP2 expression. Furthermore, GO and KEGG enrichment analysis results of IGF2BP2 co-expressed genes indicated that these genes are mainly associated with immunity/inflammation and tumorigenesis. In addition, IGF2BP2 and its co-expressed genes are associated with tumor-infiltrating immune cells (p<0.01). Conclusion: IGF2BP2 may be a potential prognostic biomarker in OSCC and correlates with immune infiltrates.

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A new reference plane of the head, based on soft tissue landmarks and suitable for clinical analysis and soft tissue measurements, is needed. We recently discovered a novel reference plane, that is, the COs plane, which was formed by bilateral Lateral Canthus and bilateral Otobasion Superius and might be horizontal at the natural head position (NHP). In this study, we investigated the angles between the COs plane and the true horizontal (TrH) plane while sitting and standing, aiming to explore whether the new plane could be used as a substitution for the reference plane in clinical practice. Briefly, 26 volunteers were enrolled. Their sitting and standing NHP were recorded using the 3dMDface system. In the pitch axis, the mean angle of COs/TrH was 0.29 degrees and 0.41 degrees for sitting and standing positions, respectively, showing no statistical difference (P > 0.05). However, in the roll axis, the mean angle of COs/ TrH for sitting position and standing positions was 1.08 and 1.33 degrees (P < 0.05), which was considered to be the result of habitual canting of the head. Moreover, there were no significant differences between the sitting and standing NHP, considering the COs/TrH angles (P > 0.05). To sum up, we concluded that the COs plane could be used as a reliable reference plane in certain soft tissue analysis and clinical applications.