Plant Growth-Promoting Bacteria (PGPB) are a promising alternative to conventional fertilization. One of the most interesting PGPB strains, among the spore-forming bacteria of the phylum Firmicutes, is Bacillus pumilus. It is a bacterial species that inhabits a wide range of environments and shows resistance to abiotic stresses. So far, several PGPB strains of B. pumilus have been described, including B. pumilus LZP02, B. pumilus JPVS11, B. pumilus TUAT-1, B. pumilus TRS-3, and B. pumilus EU927414. These strains have been shown to produce a wide range of phytohormones and other plant growth-promoting substances. Therefore, they can affect various plant properties, including biometric traits, substance content (amino acids, proteins, fatty acids), and oxidative enzymes. Importantly, based on a study with B. pumilus WP8, it can be concluded that this bacterial species stimulates plant growth when the native microbiota of the inoculated soil is altered. However, there is still a lack of research with deeper insights into the structure of the native microbial community (after B. pumilus application), which would provide a better understanding of the functioning of this bacterial species in the soil and thus increase its effectiveness in promoting plant growth.

Plant growth-promoting bacteria are one of the most interesting methods of controlling fungal phytopathogens. These bacteria can participate in biocontrol via a variety of mechanisms including lipopeptide production, hydrolytic enzymes (e.g., chitinase, cellulases, glucanase) production, microbial volatile organic compounds (mVOCs) production, and induced systemic resistance (ISR) triggering. Among the bacterial genera most frequently studied in this aspect are Bacillus spp. including Bacillus pumilus. Due to the range of biocontrol traits, B. pumilus is one of the most interesting members of Bacillus spp. that can be used in the biocontrol of fungal phytopathogens. So far, a number of B. pumilus strains that exhibit biocontrol properties against fungal phytopathogens have been described, e.g., B. pumilus HR10, PTB180, B. pumilus SS-10.7, B. pumilus MCB-7, B. pumilus INR7, B. pumilus SE52, SE34, SE49, B. pumilus RST25, B. pumilus JK-SX001, and B. pumilus KUDC1732. B. pumilus strains are capable of suppressing phytopathogens such as Arthrobotrys conoides, Fusarium solani, Fusarium oxysporum, Sclerotinia sclerotiorum, Rhizoctonia solani, and Fagopyrum esculentum. Importantly, B. pumilus can promote plant growth regardless of whether it alters the native microbiota or not. However, in order to increase its efficacy, research is still needed to clarify the relationship between the native microbiota and B. pumilus. Despite that, it can already be concluded that B. pumilus strains are good candidates to be environmentally friendly and commercially effective biocontrol agents.

Industrial hemp (Cannabis sativa L.) is a plant species cultivated as a raw material for fiber extraction. Alternatively, hemp biomass can be used for feeding or energy purposes. This study was conducted to investigate the effect of inoculation with a lactic acid bacteria starter culture on the fermentation and chemical compositions of hemp silages. Hemp shoots (HS) and hemp flowers (HF) were ensiled in mini laboratory silos without or with the inoculation of the commercial starter culture Lactosil Biogaz (Lentilactobacillus buchnerii KKP 907 p; L. buchneri A KKP 2047 p; Pediococcus acidilactici KKP 2065 p). After 7 and 42 days of ensiling, the fermentation quality and chemical compositions of the silages were assessed. The use of Lactosil Biogas for hemp resulted in a decrease in pH, increase in lactic acid (LA), and reduction in fungal abundance in the HS silage. In the case of the HF silage, the bacterial inoculation was less effective; however, an increase in LA and a decrease in butyric acid (BA) were observed. As a result of the ensilage process, decreases in crude fiber and hemicellulose were observed in the HS and HF silages. Thus, hemp ensiling with biological additives is an effective pre-treatment of hemp plants for subsequent biofuel production that can preserve the biomass and provide the year-round availability of feedstock.

Melica uniflora Retz., commonly known as wood melick, is a grass species that is native to most of Europe. Melica uniflora grows in hardwood forests near Fagus species, providing vital food and shelter for forest wildlife. The nutritional status of wood melick is not sufficiently recognized. The study aims to identify the intrapopulation variability of Melica uniflora plants collected from natural forest habitats in Poland in terms of nutritional status variability in relation to stage development and the course of weather conditions. The research was conducted for two consecutive years: 2021 and 2022, in the area of the Ślęża Massif, near the town of Sobótka in Lower Silesia voivodeship (Poland). The material for analyses was collected from 10 natural forest sites twice: in July (I) and October (II). The content of nutritive components: crude protein (CP), crude ash (CA), neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), and water-soluble carbohydrates (WSC) was evaluated. Relative feed value (RFV), dry matter digestibility (DDM), dry matter intake (DMI), cellulose (CL), and hemicellulose (HCL) content were calculated based on ADF and NDF. Melica uniflora plants contained 75.2 g·kg−1 DM of CP, 290.6 g·kg−1 DM of CF, 120.0 g·kg−1 DM of CA, and 25.9 g·kg−1 DM of WSC. The content of NDF was 637.6 g·kg−1 DM, ADF 407.5 g·kg−1 DM, and ADL 58.0 g·kg−1 DM. The nutritional status of wood melick depended on the course of weather conditions in the following years and its location, which changed during the growing season. Plants collected in the first year of the study contained more CL, NDF, and ADF fractions and less CA, ADL, and WSC. Melica uniflora plants harvested in June were characterized by higher CP (102.1 g·kg−1 DM) and WSC (30.1 g·kg−1 DM) content and lower content of remaining nutrients. It can be concluded that Melica uniflora plants can be a valuable source of these nutrients in the forage of forest animals.