In this work, the authors present experimental results of tunable amplified spontaneous emission (ASE) and random lasing (RL) emission wavelength, which mechanism is based on Rhodamine 6G (Rh6G) aggregates formation. Dye concentration controlled light amplification has been applied for RL and ASE on Rhodamine-doped biopolymeric layers like modified deoxyribonucleic acid (DNA) or starch. Additionally, measurements of ASE from water suspension with and without phosphatidylcholine (PC) lipids, confirmed Rhodamine aggregates role on different wavelength emission of ASE and RL. There have been acquired wide tuning of lasing equal to 35 nm observed for different studied biological systems.