Rice crop is mainly cultivated in large river basins which constitute unique ecosystems and their ecological quality is invaluable. Rice crop receives large amounts of pesticides whose mode of application makes soil a significant sink for pesticide residues wherein their levels should be examined. However, the high loads of pesticides used in rice cultivation contribute to the contamination of soil, water resources and in the final agricultural product. Many of the pesticides are dangerous and toxic to humans and there is a need for rapid and sensitive multi-residue analytical method as to monitor the quality of such resources.Rice cultivation demands the usage of pesticides as to be protected against herbs, fungi and insects that are causing serious problems. Rice cultivation relies on pesticide applications to ensure high yields. However, the regular use of pesticides has been found to affect the quality of neighbouring surface water systems. Thus good knowledge of the environmental fate and dissipation of pesticides in the paddy rice environment would become available. European Unions has established Maximum Residue Levels (MRL) in agricultural products (like rice) with the Regulation 396/2005 and for drinking water with the Directive 80/778/EC. The aforementioned MRL's have been established according to lawful use of pesticides as it is defined in Regulation No.1107/2009.The objective of the present study was the development, optimization and validation of multi-residue analytical methods for the determination of the main pesticides used in rice cultivation which are penoxsulam, tricyclazole, propanil and its main metabolite 3,4-dichloroaniline, azoxystrobin, molinate, profoxydim, cyhalofopbutyl and deltamethrin in soil, in surface water, and in rice.The pre-treatment techniques that have been used were solid phase extraction (SPE) for surface water samples and matrix solid phase dispersion (MSPD) for soil and rice samples.The developed and validated analytical methods were very reliable with low LOD and LOQ, good repeatability and precision (RSD% < 12%) and high recoveries for all the studied pesticides.The aforementioned analytical methods were applied in a dissipation study.Thus, laboratory and two-year field studies were performed to assess the environmental fate of two new generation rice herbicides (penoxsulam and