To provide the most efficient electrolysis process of hydrogen and oxygen generation and the electrode twain design there were studied the following:-The process of high pressure hydrogen and oxygen cyclic generation in the membrane less electrolysis systems. -The permissible ranges of voltage variation on the electrodes were determined depending on the electrochemical reactions taking place on the active electrode. -There was studied the process of hydrolysis and oxidation of the active electrode hypoferrite at the corresponding half-cycles of hydrogen and oxygen release. -There was studied the effect of variation of the distance between the active and passive electrodes onto the electrolysis process efficiency. ________________________________________________________________________________

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Four spectrally different forms of tyrosine residues were shown to be present in proteins, namely, nonionized residues, either buried or exposed to solvent, and ionized residues buried or exposed to solvent. A method for determining the pK a values of the tyrosine residues in proteins was proposed. It is based on the decrease in the absorption intensity in the second derivative of the UV spectrum at 284.2 nm, which is the wavelength of the isobestic point corresponding to the transition of the nonionized tyrosine residues from the buried to the exposed state. Several proteins were studied by this method; the results obtained were found to be close to the corresponding pubtished data. This method is simpler than the conventional LW titration.Key words: ionization of tyrosine in proteins, UV absorption spectra, second derivative.Information on the dissociation constants of tyrosine residues in proteins is important for the investigation of the microenvironment of these groups and the structure of the protein as a whole in solution. These constants are normally found from the variation of the intensity of the absorption of ionized tyrosine residues in proteins as a function of the pH at a wavelen~h of 293--295 nm, i.e., near the absorption maximum of these groups, which is shifted 8--10 nm to longer wavelengths with respect to the absorption maximum of the nonionized residues, t In some cases, NMR spectroscopy is used; this makes it possible to determine pK a of the tyrosine groups in a protein from the dependence of the chemical shift of the carbon atoms in the aromatic ring on the pH of the medium, zRecently, even derivatives of the absorption intensity with respect to wavelength have been widely used to interpret spectral data. Their advantage over the ordinary spectrum is that the half-widths of peaks are much smaller. This makes it possible to distinguish peaks that overlap in the ordinary spectrum. For example, the use of the second and fourth derivatives in the studies of the UV spectra of proteins makes it possible to distinguish the absorption peaks of individual aromatic amino acids. The minimum in the second derivative of the spectrum and the maximum in the fourth derivative coincide in wavelength with a maximum or a shoulder in the initial spectrum, respectively. 3-sWe assumed that, owing to the high resolution of peaks, the even derivatives of the spectrum would also be convenient for the investigation of the ionization of tyrosine residues in proteins. In this study, we used the second derivative of the UV spectrum (SDUS), since in higher-order derivatives, peaks of ionized tyrosine residues cannot be distinguished from the background. 5 In the fourth derivative of the spectrum and in higherorder derivatives, the intensity of the peaks of ionized tyrosine residues is negligibly small due to the large halfwidths of these peaks in the initial spectrum (dnA/dk n = K'A/F n, where F is the half-width of a peak in the spectrum and K is a constant).In the present study, we are proposing and using a method for...

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