Изучена динамика электрофоретической подвижности эритроцитов, агрегация эритроцитов, концентрация в них малонового диальдегида и клинико-лабораторные показатели эритроцитов при применении цитофлавина (0,2 мл/кг/сут в течение 7 сут) у крыс с экспериментальной артериальной гипертензией. Результаты исследования показали, что при действии препарата на 7 сут после его введения регистрировалось повышение электрофоретической подвижности эритроцитов, снижение агрегации и концентрации малонового диальдегида в эритроцитах, уменьшение количества эритроцитов, гемоглобина, гематокрита, среднего объема эритроцита и содержания гемоглобина в одном эритроците относительно соответствующих показателей группы животных с артериальной гипертензией. Влияние цитофлавина вызывало снижение систолического артериального давления на 7 сут исследования при тенденции к снижению диастолического артериального давления. Выявленные эффекты препарата позволяют расширить возможности его терапевтического использования в качестве средства, корригирующего микрореологию крови при артериальной гипертензии.

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It is shown that exposure of rat erythrocytes to low doses of ionizing radiation (0.04, 0.08, 0.16, 0.25, and 0.33 mGy) leads to nonlinear changes in lipid peroxidation processes in the membrane of erythro cytes, their electrophoretic mobility, and osmotic resistance. Ionizing radiation in the dose range from 0.08 to 0.16 mGy apparently triggers apoptosis, leading to deceleration of hemolysis and stabilization of erythro cytes, which was confirmed by morphological changes in erythrocytes.

Purpose.To evaluate the role of ozonized erythrocytic mass transfusion in the restoration of damages in the architectonics of myocardium microvasculature and cardiomyocytes in case of a severe blood loss.Materials and Methods. Two batches of experiments were conducted, 17 white outbred rats in each. The animals were anesthetized with thiopental sodium (25 mg/kg). Blood loss was caused by taking 3ml of blood from the tail artery of rats, which is 35% of the circulating blood volume. One hour after the blood loss, transfusion of autoerythrocytes with normal saline solution and Ringer’s solution infusion in 1:1 ratio was performed in the control batch. In the experimental batch, 3 ml of autoerythrocytes treated with ozonized saline solution with ozone concentration of 2 mg/l and 3 ml of Ringer’s solution (1:1 ratio) were administered to rats. Erythrocyte mass was prepared from 3 ml of the autoblood harvested from the animals 3 days before the experiment. On a post-transfusion day5, in both batches and in five intact animals, hearts were removed following the intraperitoneal thiopental sodium injection (100 mg/kg). The left ventricle wall specimen from each heart was removed for examination. Histologic sections were stained with hematoxylin and eosin. The preparations were visually examined and morphometric studies were performed using microvisor Vizo-103.Results.It has been established that administration of ozonized erythrocyte for severe blood loss correction limited the decrease in numbers of capillary profiles and their diameters, formation of mixed and hyaline thrombi fully or partially occluding microvascular lumens, major hemorrhages and reduction of variation of nuclear profiles, decreased perivascular, pericellular, perinuclear, and endonuclear edema of myocardial tissue, cardiomyocyte overcontruction zone, and their ruptures.Conclusion.The positive trends for microcirculation indices, vascularization density and myocardial edematization as prognostic markers in assessing potential posthypoxic rehabilitation of damaged tissues upon blood loss correction with ozonized erythrocyte mass might be explained by the antihypoxic, antioxidant, and detoxifying actions of ozone on erythrocytes and/or its metabolites (ozonides) in the body post-transfusion.