The work was carried out molecular-epidemiological analysis of HIV-1 in the cities of the North-West Federal District — Vologda and Cherepovets. The study used a collection of peripheral blood mononuclear cells (PBMC) obtained from 80 HIVinfected patients: 52 samples were obtained from patients living in Cherepovets, and 28 samples — from Vologda. The distribution of the HIV-1 genetic variants in the studied cities was as follows: sub-subtype A6 — 51,25%; subtype B — 6,25%; the recombinant form of CRF_03AB — 32,5%; unique recombinant forms (URFs) — 6,25%, and 3,75% were represented by other subtypes: G and CRF63_02A1. A phylogenetic analysis confirmed the relationship of the sub-subtype A6 viruses with the A6 (IDU-A) variant predominating in Ukraine, Russia and other former Soviet Union (FSU) countries; the sequences of subtype B formed a common branch on the phylogram with reference strains characteristic of men who have sex with men; 32,5% of the nucleotide sequences formed a single cluster with the reference strain CRF03_AB. In addition to these subtypes, the presence of unique recombinant forms of HIV-1 containing segments of the sub-subtype A6 and IDU-B viruses were also found. The results of the molecular epidemiological analysis in the Vologda Oblast also showed significant differences in the genetic profile of HIV-1 in two nearby cities — Vologda and Cherepovets. Thus, the evolution of HIV-1 in the Vologda Oblast continues, with the main source of variability being the mutual penetration of viruses between risk groups and recombination processes.

Purpose. To analyze the epidemiological situation and perinatal HIV infection in the Vologda region.Materials and methods. The article provides a retrospective and prospective analysis of data obtained from the forms of state statistical observation and accounting forms of medical documentation, using the Chi-square calculator, Fisher criterion, risk ratio to assess the effectiveness of measures to prevent perinatal HIV transmission in the Vologda region.The results of the study showed that the detection of HIV infection during pregnancy in the Vologda region tends to increase. At the same time, there are still problems such as adherence to dispensary monitoring and treatment in women who have drug dependence, which requires further improvement of the legal framework and clear implementation of approved algorithms by medical professionals.