We studied parameters of lipid peroxidation (LPO) in women with infertility, resident of the Republic of Buryatia. The levels of malonic dialdehyde (MDA) were increased in the examined infertile women from Buryat and Russian populations. Reduced total antioxidant activity, activity of superoxide dismutase (SOD), and α-tocopherol levels were revealed in patients from Buryat population; decreased concentrations of reduced glutathione were recorded in patients from Russian populations.

LPO processes and blood antioxidant status were studied in reproductive-age women with the hypothalamic syndrome. The control group consisted of somatically healthy women without reproductive disorders. Female patients with the hypothalamic syndrome were characterized by an imbalance in the LPO-antioxidant defense system, which aggravated the course of the underlying disease.

Materials and methods. The article presents an assessment of the influence of factors on the effectiveness of cryoprenoses. Scientists conducted a retrospective analysis of 149 cryogenic transfer on the basis of the department of Auxiliary reproductive technologies of the Irkutsk Regional Perinatal Center for 2017. Patients signed voluntary consent to participate, the patients were divided into 4 groups, depending on the duration of storage of embryos: group 1 - 57 people, with a shelf life of 1-3 months, group 2 - 29 people, storage period 4-6 months, group 3 - 25 people, the shelf life is 7-11 months, group 4 - 38 people, the shelf life is 12 months or more. The average age of women is 30.69±2.9 years. Selection criteria for research: tubal factor of infertility, frozen embryos. Results. The analysis of cryopreference showed that the age of patients, the quality of transferred devitrified embryos and the duration of storage of cryopreserved embryos have a more important influence.

The purpose of this study was to assess the association of two polymorphic loci Ile105Val/Ala114Val of the GSTP1 gene with the parameters of oxidative stress (OS) in men with infertility. We retrospectively analyzed the results of a survey of 222 men (mean age of 29.9±5.3 years) of infertile couples. A control group of 104 men (30.2±3.6 years) was formed, consisting of healthy men with realized reproductive function. An analysis of the frequency with which the Ile105Val polymorphism of the GSTP1 gene occurred in men with infertility and in fertile men found statistically significant differences (χ 2 =7.487; P=0.024). When comparing the frequency distribution of the genotypes of the Ala114Val polymorphism of the GSTP1 gene in infertile men and fertile patients, no significant differences were found between the groups (χ 2 =3.823; P=0.14). In men with infertility, carriers of the heterozygous genotype of the GSTP1 Ile105Val polymorphism presented an increase in reduced glutathione activity by 7% (P=0.0004), a decrease in glutathione reductase activity by 20% (P=0.03) in serum, and a decrease in SOD activity by 8% (P=0.01) in the ejaculate, unlike fertile men with heterozygous polymorphism, who had an increase in the total antioxidant activity of the blood serum by 20% (P=0.0001) and a decrease in glutathione peroxidase activity by 24% (P=0.03) in the ejaculate. In men with infertility, carriers of the heterozygous genotype of the GSTP1 Ala114Val polymorphism presented a decrease in α-tocopherol concentration by 15% (P=0.002), an increase in glutathione peroxidase activity by 25% (P=0.0004) in the blood and a decrease in SOD activity by 7% (P=0.01) in the ejaculate, unlike fertile men with heterozygous polymorphism, who had an increase in the concentration of serum conjugated dienes by 19% (P=0.0001) and a decrease in glutathione-S-transferase activity by 32% (P=0.03) in the ejaculate. Carrier identification of the GSTP1 Ile105Val and Ala114Val polymorphic loci, as well as the determination of the enzymes of the thiol-disulfide system, can be recommended for an additional assessment of the risk of developing reproductive disorders in men.

The aim of this research was to study the concentration of lipid peroxidation (LPO) products and the activity of antioxidant defense (AOD) parameters in ISIAH rats in comparison with a group of adolescents with arterial hypertension (AH). Material and Methods:We conducted the study on young (2.5-3 months) sexually mature male rats of the normotensive line (WAG) (n=20) (intact animals) and hypertensive line (ISIAH) (n=20), weighing 200-220 g. The data of 65 adolescents aged 13-17 years with AH (Group 1) were used for the clinical study (the age of adolescents is comparable to the biological age of the experimental animals used). The comparison group consisted of 65 normotensive adolescents of the same age and sex ("copypair" type) (Group 2). The plasma level of antioxidant parameters (total antioxidant activity [TTA], SOD activity, α-tocopherol and retinol) and primary/secondary products of LPO (conjugated dienes [CD], ketodienes and conjugated trienes [KD-CT], and thiobarbituric acid reactants [TBARs]) were determined using spectrophotometric and fluorometric methods.Results: We found that the course of LPO reactions in animals and humans was similar, which was expressed by the activation of prooxidant factors and the insufficiency of antioxidant response. Species differences concerned the intensity and number of parameters involved in the pathological process. Thus, in ISIAH rats there was an increase in toxic TBA-active products and a decrease in TTA, α-tocopherol and retinol in comparison with normotensive animals; in AH adolescents there was an increase in the content of intermediate-KD-CT and final TBA-active products, and a decrease in the α-tocopherol level in relation to the comparison group.Conclusion: Features of response of the LPO nonspecific system in hypertensive rats and humans allow using this line of rats for further study of adaptive mechanisms, and to extrapolate the received experimental data on humans, taking into account certain specific distinctions.(International Journal of Biomedicine. 2019;9(4):292-296.)